A total thirteen strains of Pseudomonas spp. were isolated from patients and hospital environment and were identified as a Pseudomonas aeruginosa. All isolated strains were shown siderophore production on chromo azural S agar plate. The maximum siderophore production was found in strain PAUTI4 i.e. 67%. Evaluation study of siderophore shown that maximum siderophore were obtained on succinate medium at 7.0 pH with iron concentration 10µM.

The DNA binding protein of stationary phase cells (Dps)-first discovered in starved Escherichia coli-is capable of providing protection to cells during exposure to various environmental assaults. Its ability to do so is based on three intrinsic properties of the protein: DNA binding, iron sequestration, and its ferroxidase activity. Proteomic studies have lead to the inference of a regulatory role for Dps as well; however, the ability of Dps to serve as a global regulator during nutritional deprivation has yet to be directly examined. In this study, we utilized microarray analysis and quantitative real-time PCR to establish direct evidence for a regulatory role of Dps in starved Salmonella enterica serovar Enteritidis. The results of our microarray screening revealed over 150 genes significantly up or down regulated in starved cells lacking functional Dps protein. Also, we identified a small subset of genes regulated by Dps that are important for the induction of hydrogen peroxide, iron, and acid resistance. The fact that it positively regulates genes important for stress resistance further solidifies Dps as a virulence regulator in S. enteritidis; for resistance to such cytotoxic conditions is likely to translate into enhanced survivability and virulence within infected hosts.

A total of 25 different types of actinomycetes were isolated from the soils of Agricultural University. All the isolated actinomycetes were characterized and identified based on the morphological, biochemical, cultural characteristics .The isolates were belonged to Streptomyces, Micromonospora and Nocardia species. Optimization for the growth of all the isolates was carried out in batch culture. Strain was cultured on the basal medium with different carbon and nitrogen sources and their effect on the growth was studied. Both primary and secondary screening methods were used to screen actinomycetes for antibacterial activity. The result of the screening revealed that all the isolates were against bacterial culture. But the best strain was found to be Streptomyces sp as they showed broad spectrum activity with big zone of inhibition, after mutation, the strain Streptomyces sp showed increased antibacterial activity against all the tested human bacterial pathogens. The crude extract were further analysed by thin layer chromatography on silica plates. The Rf values in the TLC chromatograph further characterize that Streptomyces sp 2comes under aminoglycoside group, very much related to Streptomycin and the Streptomyces sp 3 comes under the antibiotic cephalexin. HPLC profile of the antimicrobial compounds of Streptomyces sp were performed by Rheodysne Column (C-18) up to 10 min at 220 nm. The peaks which had antimicrobial activity of Streptomyces sp1 was identified to be at 3.296. This is similar to oxohexaene antibiotic whereas, the antimicrobial compound of Streptomyces sp3 showed a retention time of 3.233 on HPLC, this peak was similar to cephalexin.

Semiconductive nanoparticles of bacteria as Bacillus subtilis with catalyst MgFe₂O₄ biofilm was synthesized by using solution combustion technique. The process was convenient, environment friendly and efficient method. Materials were characterized by TG/DTA, XRD, and TEM. Thick biofilm of Bacillus subtilis MgFe₂O₄ was measured by exposing it to reducing economical gases. It was found that the Bacillus subtilis was sensors exhibited various sensing responses to these gases at different operating temperature. The sensor exhibited a fast response and a good recovery. The biofilm can be used as a new type of gas-sensing material which has a high sensitivity and good selectivity to various gases at low ppm.