Comparative study conducted in the Department of Microbiology in a tertiary care teaching hospital during December 2019 to May 2021. Minimum inhibitory concentration of colistin determined by broth microdilution method for clinical isolates of Carbapenem resistant Enterobacteriaceae (CRE)and compared with the results obtained in Vitek 2 Compact. A total of 205 CRE isolates were included in the study. Klebsiella pneumoniae (60.5%) was the predominant CRE isolate, followed by E. coli (36.6%) and Enterobacter cloacae (2.9%). MIC ranging from 0. 0625 µg/ml to 0.5 µg/ml were obtained for our study isolates. All the 205 Carbapenem resistant Enterobacteriaceae isolates in the study were found to be susceptible to colistin both by BMD method and VITEK 2 method. There was 100% categorical agreement between microbroth dilution method and VITEK 2 system for colistin susceptibility. Our study results showed that automated method, VITEK 2 compact system can be used as reliable alternative method for reporting colistin susceptibility