The strain of Corynebacterium glutamicum was tested on nutrient agar plate for its purity. This strain was further used for glutamic acid production under optimum growth conditions. Studies revealed that whole cells produce more glutamic acid compared to immobilized cells. It was also observed that among immobilized cells agarose produces more glutamic acid as compared to alginate. It was concluded that immobilized cells are more beneficial that whole cells as they are reusable and avoids chances of contamination hence cost effective.

Enzymes play a vital role in the industry with a wide range of application. Agricutural waste is the maximum waste been produced in India. This Project aims at using various agricultural wastes for the production of Protease. To achieve this various research is being conducted. Various Bacterial and Fungal species are being used for the production of Protease. Penicillium species was being used in this work for the production of Protease .In this work the various components of the media were studied and were optimized and used for the production of Protease.

Candida albicans is an opportunistic commensal of the human gastrointestinal tract and vaginal mucosa, causing opportunistic fungal infections in an immunocompromised patient. In the present study we have investigated the effect of ascorbic acid on growth and its several pathogenicity markers. Turbidometric measurement for growth; proteinase assay, WST-1 cell cytotoxicity assay, colony count method and inverted microscopy were performed to check pathogenecity markers of C. albicans ATCC 10261 strain. 150 mg/ml concentration of ascorbic acid arrests cell growth. It was observed that higher ascorbate level of 250 mg/ml reduces proteinase secretion (an important mechanism suggestive of virulence in Candida) exhibited by mean precipitation zone value of 2.375 which is remarkably less than that of Control cells (value 4.125). At higher concentration of ascorbic acid increases cell cytotoxcity (79.71 percent inhibition at 150 mg/ml) and decreases percent viability under oxidative stress (98 percent reduction at 250 mg/ml concentration). Transition studies showed cessation of germ tube induction and hyphae formation at lower concentrations (15 mg onwards) of ascorbic acid. Results indicate that higher ascorbic acid level somehow decreases pathogenic attribute of Candida albicans, while yeast to hyphal studies show an exception, were lower concentration was effective in inhibiting hyphae formation. Thus ascorbic acid exhibits its pro-oxidant nature in present in-vitro studies.

In India antibiotics are frequently used for preventing and controlling bacterial pathogens in carp aquaculture system, yet no studies have been performed to evaluate the ecological impact of its intensive and prolonged use. In this work the frequency of oxytetracycline-resistant bacteria from water, palletized feed and different life stages of fish from Indian freshwater carp aquaculture system as well as the level of resistance of selected strains was investigated. Viable as well as antibiotic-resistant bacterial counts were performed by spread plate method in culture media supplemented with the oxytetracycline. Sixty two resistant Gram negative isolates which represented the oxytetracycline-resistant bacterial population, were randomly selected on nutrient agar supplemented with oxytetracycline (50μg/ml) from carp farms and feed pellet samples. Among these bacterial isolates Flavobacterium (21%), Alcaligenes (14.5%), Aeromonas (11%), Pseudomonas (10%) and Enterobacteriace (19%) were the most frequent. The Escherichia, Serratia, Citrobacter, Enterobacter, Shigella and Proteus from Enterobacteriace were recovered. Twelve isolates of oxytetracycline resistant bacteria were mainly dominated in adult fishes by the genus Flavobacterium (23%) and Enterobacteriace(41%). Selected strains exhibited high levels of oxytetracycline resistance with minimum inhibitory concentrations (MICs) ranging from 50 to 600μg/ml.This study shows the presence of an important population of oxytetracycline-resistant bacteria in the microflora of Indian carp aquaculture farms. Therefore the environment of these farms might play important roles as reservoirs of bacteria carrying genetic determinants for high level tetracycline resistance, prompting an important risk to public health.