The adolescence is phase of rapid growth and development where there is great deal of change taking place like physically, socially and emotionally. The major causes of death, disease and disability for young people have been identified as vehicular accident, suicide and unintentional injuries. But this is just a tip of ice berg, major health hazards like chronic illness and diseases are commonly caused by exposure to risk factors long before the onset of illness. It is the behavior pattern that is established during adolescence period which makes individuals susceptible to a number of illnesses that develop later in life.

Stress affects people of all ages and in all walks of life. Children at play, students preparing for an examination or workers on the job are all subject to stress. The present study was performed on 20 Adult male albino rats, out of which 10 served as control and 10 were exposed to immobilization stress. After 45 days the histological study showed that the stress has inhibitory effect on the epididymis. The degenerative histological findings are found in form of reduce number of spermatozoa, debris of cell mass & reduce epithelial cells height. These changes may cause infertility. The aim of present study was to establish the fact that stress is one of the causes of infertility.

Tumor cells have an altered amino acid metabolism. They express high levels of tryptophan and L-arginine metabolizing enzymes. This leads to depletion of tryptophan and L-arginine and locally blocks T cell proliferation. Therefore, the characteristic energy metabolism of tumor cells leads to immune suppression and contributes to immune escape processes at the tumor site [1]. Excess dietary Tryptophan has shown to cause increased tumor incidence in tumorous strains of Drosophila [2]. Canton (wild type) flies were exposed to 10mM, 20mM, 30mM, 40mM and 50mM concentrations of Tryptophan. Phenotypic analysis of the exposed flies and qualitative analysis of the isolated DNA was done 24 hours and 48 hours after exposure to Tryptophan. The quality of the DNA was evaluated using Nanodrop and the DNA was subjected to Fragmentation assay to study the damage induced. Phenotypic changes observed were elongated abdomen with distinct curling and discoloration of thorax to mild orange. The isolated DNA was found to be of good quality and the fragmentation assay showed patterns of shearing. As the damage observed was not significant and the tumor induction was not observed in the experimental concentrations, tryptophan was considered non tumorigenic at the above said concentrations. However, higher concentrations of Tryptophan may induce tumor.