Plasmodium falciparum is causative agent of malaria. Peptide fragments of antigen protein can be used to select nonamers for use in rational vaccine design and to increase the understanding of roles of the immune system in infectious diseases. Analysis shows MHC class II binding peptides of antigen protein from Plasmodium falciparum are important determinant for protection of host form infection. In this assay, we used PSSM and SVM algorithms for antigen design and predicted the binding affinity of antigen protein having 224 amino acids, which shows 216 nonamers. Binding ability prediction of antigen peptides to major histocompatibility complex (MHC) class I & II molecules is important in vaccine development from Plasmodium falciparum.

Mycobacterium tuberculosis causes tuberculosis leading to be an obligatory step in infection. Peptide fragments of antigen can be used to select nonamers for use in rational vaccine design and to increase the understanding of roles of the immune system in bacterial diseases. Analysis shows MHC class II binding peptides of antigen from Mycobacterium tuberculosis are important determinant for protection of host form tuberculosis infection. In this assay, we used PSSM and SVM algorithms for antigen design and predicted the binding affinity of antigen protein having 338 amino acids, which shows 330 nonamers. Binding ability prediction of antigen peptides to major histocompatibility complex (MHC) class I & II molecules is important in vaccine development from Mycobacterium tuberculosis antigen.