Background- Aim of the present study was to explore the association of two single nucleotide polymorphisms (SNPs), including rs4956403 (SNP-1) and rs3806798 (SNP-2) located in the 5’ UTR region of interleukin 15 gene in rheumatoid arthritis of Pakistani patients. Interleukin-15 is one of the key cytokines in the pathogenesis of rheumatoid arthritis. The genetic polymorphisms located in the regulatory regions of interleukin-15 gene might play a role in rheumatoid arthritis. Methodology- Fresh blood samples were collected from different hospitals. Allele specific PCR was employed for the analysis of these polymorphisms. Results were analyzed statistically for any significant association of these polymorphisms with rheumatoid arthritis. Results- The results of our study showed that SNP-1 and SNP-2 located in interleukin-15 gene are not associated with rheumatoid arthritis in our patients. Conclusion- Any other Single nucleotide polymorphisms (SNPs) located in the regulatory and untranslated regions of IL15 gene may have association with rheumatoid arthritis, for which large scale data is required. Other molecules implicated in the inflammatory pathway of Rheumatoid Arthritis needs to be investigated.