The present study aimed to clarify the protective effect of ethanolic extract of ginger on Triton WR-1339 induced hyperlipidemia in rats. Forty male rats were randomly divided into four groups: control, Triton WR-1339 treated group received 250 mg/kg b.wt intraperitoneally (3 times per week), ginger treated group received ethanolic extract of ginger 400 mg/kg b.wt orally and ginger pretreated Triton WR-1339 group received Triton WR-1339 and ethanolic extract of ginger at the same doses for three weeks. Hyperlipidemic rats exhibited significant increase in serum total lipids, cholesterol, triacylglycerol, low density lipoprotein and very low density lipoprotein levels with reduction in serum high density lipoprotein level. Also, serum testosterone, progesterone, estradiol, triiodothyronine and thyroxine hormones, and aspartate transaminase, lactate dehydrogenase, creatine kinase and creatine kinase-MB isoenzyme activities, and Troponin I level were significantly increased. Cardiac malondialdehyde level was increased while glutathione-S-transferase, glutathione peroxidase, and glutathione reductase activities, and reduced glutathione level were significantly decreased. Treatment with ethanolic extract of ginger significantly reduced serum lipid levels and normalized serum biomarkers of cardiac functions, increased serum endocrine hormones and antioxidant enzymatic activities with subsequent decrease of malondialdehyde level. We concluded that ethanolic extract of ginger has a protective role against cardiac damaging effect of hyperlipidemia.