Microarray is new techniques to investigate the expression levels of thousands of genes simultaneously. Image analysis is an important aspect of microarray experiments, one, which can have a potentially large impact on subsequent analysis such as clustering or the identification of differentially expressed genes. Microarray image spot position & size determination is a very important step in the analysis of microarray image because it is the first part we need to do for the analysis. By comparing gene expression in normal and abnormal cells, microarrays may be used to identify genes, which are involved in particular diseases. These genes may then be targeted by therapeutic drug. Making this part automated and fast is also important. So we consider this as indeed problem of correct spot detection and automatic counting. Overcome it by using flat structuring element (disk, diamond, square and octagon shaped) morphological segmentation technique, which gives satisfied results. And also we compare the original & resultant image results on the basis of different image quality measure.

The Indian economy mostly depends on agriculture. But the farmers in leading state like Maharashtra are committing suicides because of environmental parameters which strongly affect the crops. This forced us to think in the direction of farming and we mood to the topic mentioned below inspired by Eden project in UK and BIOS-3 project in Russia. Greenhouse provided up to present era is not completely automatic and there are many errors and limitations in its working and are unaffordable, large, difficult to maintain and less accepted by the technologically unskilled workers. So we are trying to provide completely automised CES (Closed Ecological System) using runtime TCP-IP protocol to monitor and control the parameters which governs the plant growth. The architecture of a green house monitoring system comprises of a set of sensor nodes and a control unit that communicate with each sensor node and collects local information to make necessary decisions about the physical environment. The user is updated with the run time information of his field.