Title |
MAGNITUDE OF THYMINE IN DIFFERENT FRAMES OF MESSENGER RNAs |
| Int J Bioinformatics Res Vol:4 Iss:3 (2012-08-06) : 273-275 |
Authors |
RAJASEKARAN E., ASHA JACOB, KLAUS HEESE |
Published on |
06 Aug 2012 Pages : 273-275 Article Id : BIA0000328 Views : 1086 Downloads : 1213 |
DOI | http://dx.doi.org/10.9735/0975-3087.4.3.273-275 |
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Abstract |
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Thymine is the one and only base transcribed into uracil during production of proteins. Thymine in DNA and uracil in mRNA plays a major role in producing proteins with appropriate carbon content for stability and activity. Thymine distribution is different frames of coding nucleic acids are investigated statistically. The results confirm that frame 1 supposed to have definite thymine content. Frame 3 prefers to have least thymine content. Frames 4 & 5 maintain some degree of thymine while 2 & 6 have a variable fraction of thymine.
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Title |
IN-SILICO APPROACH FOR PREDICTION OF VACCINE POTENTIAL ANTIGENIC PEPTIDES FROM 23-kDa TRANSMEMBRANE ANTIGEN PROTEIN OF Schistosoma haematobium |
| Int J Bioinformatics Res Vol:4 Iss:3 (2012-08-13) : 276-281 |
Authors |
GOMASE V.S., CHITLANGE N.R., CHANGBHALE S.S., SHERKHANE A.S., KALE K.V. |
Published on |
13 Aug 2012 Pages : 276-281 Article Id : BIA0001579 Views : 1087 Downloads : 1214 |
DOI | http://dx.doi.org/10.9735/0975-3087.4.3.276-281 |
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Abstract |
Full Text |
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CNKI |
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Open Access |
Urogenital schistosomiasis is frequently occurring parasitic disease in tropical countries, S. haematobium is main causative agent responcible for urogenital schistosomiasis; till date no effective invention made to against urogenital schistosomiasis. In this analysis we a have predicted suitable antigenic peptides from Schistosoma haematobium 23-kDa transmembrane protein for peptide vaccine design against urogenital schistosomiasis based on cross protection phenomenon as, an ample immune response can be generated with a single protein subunit. We found MHC class II binding peptides of S. haematobium 23-kDa are important determinant against the diseased condition. The analysis shows S. haematobium 23-kDa transmembrane protein having 218 amino acids, which shows 210 nonamers. In this assay, we have predicted MHC-I binding peptides for 8mer_H2_Db allele (optimal score is 14.128), 9mer_H2_Db allele (optimal score is 20.065), 10mer_H2_Db allele (optimal score is 13.776), 11mer_H2_Db allele (optimal score is 31.213). We also predicted the SVM based MHCII-IAb peptide regions, 152-DYGPNIPAS, 51-WQAAPIAII, 50-VWQAAPIAI, 142-FHCCGAKGP, 97-AELAAAIVA (optimal score is 14.911); MHCII-IAd peptide regions, 100-AAAIVAVVY, 71-LGCCGAIKE, 192-FGVCFFQLL, 186-IVACVAFGV (optimal score is 13.112); and MHCII-IAg7 peptide regions 42-QYGDNLHKV, 101-AAIVAVVYK, 28-VLIGAGAYV, 103-IVAVVYKDR, 203-VIACCLGRQ (optimal score is 11.605) which shows potential binders from S. haematobium 23-kDa transmembrane protein. The method integrates prediction of MHC class I binding proteasomal C- terminal cleavage peptides and Six potential antigenic peptides at average propensity 1.094 having highest local hydrophilicity. Thus a small antigen fragment can induce immune response against whole antigen. This approach can be applied for designing subunit and synthetic peptide vaccines.
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