VITAMIN E AS AN ANTIOXIDANT ON MEMBRANE INTEGRITY OF CRYOPRESERVED SPERM IN DIFFERENT BREEDS OF BULLS

D. PANDA¹, A.P.K. MAHAPATRA²*, S. MOHAPATRA³, N. DALAI⁴, S.R. MISHRA⁵, A.K. KUNDU^6
1Department of Veterinary Physiology, College of Veterinary Science & Animal Husbandry, Odisha University of Agriculture and Technology, Bhubaneswar, 751003, Odisha, India
²Department of Veterinary Physiology, College of Veterinary Science & Animal Husbandry, Odisha University of Agriculture and Technology, Bhubaneswar, 751003, Odisha, India
³Department of Veterinary Physiology, College of Veterinary Science & Animal Husbandry, Odisha University of Agriculture and Technology, Bhubaneswar, 751003, Odisha, India
⁴Department of Veterinary Physiology, College of Veterinary Science & Animal Husbandry, Odisha University of Agriculture and Technology, Bhubaneswar, 751003, Odisha, India
⁵Department of Veterinary Physiology, College of Veterinary Science & Animal Husbandry, Odisha University of Agriculture and Technology, Bhubaneswar, 751003, Odisha, India
⁶Department of Veterinary Physiology, College of Veterinary Science & Animal Husbandry, Odisha University of Agriculture and Technology, Bhubaneswar, 751003, Odisha, India
* Corresponding Author : ambika28@hotmail.com

Received : 09-09-2023     Accepted : 27-10-2023     Published : 30-10-2023
Volume : 15     Issue : 2       Pages : 959 - 961
Genetics 15.2 (2023):959-961

Keywords : Vitamin E, Antioxidant, Cryopreserved sperm, Membrane integrity, Sperm motility
Academic Editor : Dr Jalajakshi, I S Chakrapani, Prof Dr Suleyman Cylek, Dr Pravin Suryakant Bankar
Conflict of Interest : None declared
Acknowledgements/Funding : Authors are thankful to Deputy Director, Frozen Semen Bank, Khapuria, Cuttack, and Department of Veterinary Physiology, College of Veterinary Science & Animal Husbandry, Odisha University of Agriculture and Technology, Bhubaneswar, 751003, Odisha, India
Author Contribution : All authors equally contributed

Aim: To study the effects of Vitamin E in different dose on the semen parameters during cryopreservation processes in different breeds of bulls. Material and Methods: Three different breeds of healthy bulls; Red Sindhi (CB) (n=6), Haryana (CB) (n=6) and Jersey (CB) (n=6) with same feeding practice belonging to the frozen semen bank, Khapuria, Cuttack, Odisha were selected for the study. In this study, four types of extender with different concentration of vitamin E (2.5mM, 5mM and 10mM) were used. Four types of straws were prepared and all these straws underwent programmed freezing (-196°C). After cryopreservation, straws were thawed in water bath maintained at a temperature of 37°C for 15 minutes. Then different tests were conducted like individual motility, live and dead count, normal abnormal count and acrosomal integrity test for different straws. Results: The effects of vitamin E on different sperm parameters like individual motility, live and dead, acrosomal integrity, and normal and abnormal percentage in dose wise (normal, 2.5, 5, 10 milli mole) varied significantly. Vitamin E at 5 milli mole shows maximum result while 2.5 milli moles have least result. Conclusion: On the basis of the present experiment, we can conclude that the dose wise effect of vitamin E is significant. Among all parameters, sperm motility comparatively increased after adding vitamin E at the dose rate of 5 mM/ml

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