EFFICACY OF BIOGENIC SYNTHESISED SILVER NANOPARTICLES ON BHAVIOURAL CHANGES OF Helicoverpa armigera

M.S. ISHWARYA¹, S. LOKESH²*
¹Department of Biotechnology, University of Mysore, Mysuru, 570 005, Karnataka, India
²Department of Biotechnology, University of Mysore, Mysuru, 570 005, Karnataka, India
* Corresponding Author : boramma@rediffmail.com

Received : 01-03-2022     Accepted : 27-03-2022     Published : 30-03-2022
Volume : 7     Issue : 1       Pages : 150 - 155
J Crop Sci 7.1 (2022):150-155

Keywords : Helicoverpa armigera, Biosynthesized silver nanoparticles, Leucas aspera, Cynodon dactylon, Cyperus rotundus, Antifeedant, Larvicidal, Gut protease
Conflict of Interest : None declared
Acknowledgements/Funding : Authors are thankful to Department of Biotechnology, University of Mysore, Mysuru, 570 005, Karnataka, India
Author Contribution : All authors equally contributed

The grounds of this study were to utilize the biogenic synthesized silver nanoparticles from Leucas aspera, Cyperus rotundus and Cynodon dactylon for the control of polyphagous pest Helicoverpa armigera. Synthesized silver nanoparticles were subjected for different characterization like XRD, EDX, FTIR and Raman spectroscopy. The bioassays (i.e., larval weight and survival rate) and also its interaction with gut protease activity (trypsin and chymotrypsin) was evaluated employing LA, CR, and CD AgNPs, as well as crude plant extracts against the 4th instar larvae. The stretching vibration of distinct functional groups of the samples was demonstrated by the existence of FTIR measurements. XRD graphs confirm the AgNPs crystallize in centered cubic phase and the change in peak intensity with different plant extracts was noticed. The body mass, length of the larvae was dramatically lowered by the synthesized sample of silver nanoparticles. They also suppressed the activity of gut proteases (trypsin and chymotrypsin). Our studies could aid in the development of new approaches to address insecticidal resistance in H. armigera

1. De Oliveira J.L., Campos E.V.R., Bakshi M., Abhilash P.C., Fraceto L.F. (2014) Biotechnol. Adv., 32, 1550-1561.
2. Lade B.D., Gogle D.P., Nandeshwar S.B. (2017) J. Nanomed. Res. 6, 1-9.
3. Randall C., Hock W., Crow E., HudakWise C., Kasai J. (2013) National Pesticide Applicator Certification Core Manual. National Association of State Departments of Agriculture Research Foundation, Washington, DC, pp. 1-17.
4. Kamaraj C., Rahuman A.A., Bagavan A. (2008) Parasitol. Res., 103, 1361-1368.
5. Kamaraj C., Rahuman A.A., Bagavan A. (2008) Parasitol. Res., 103, 325-331.
6. Senthil-Nathan S. (2015) Role of. Green Technologies. Springer- Verlag, 49-63.
7. Sasson Y., Levy-Ruso G., Toledano O., Ishaaya I. (2007) Springer, Berlin, 1-32
8. Campolo O., et al., (2017) Scientific Reports, 7 (1), 13036.
9. Gruère G., Clare N., Linda A. (2011) J. Int. Food Policy Res. Inst., 1, 35.
10. Worrall E, Hamid A, Mody K, Mitter N, Pappu H. (2018) Agronomy, 8(12), 285
11. Huang J., Li Q., Sun D., Lu Y., Su Y., Yang X., Wang H., Wang Y., Shao W., He N., Hong J. and Chen C. (2007) Nanotechnology, 18, 105104.
12. Fouad H., Hongjie L., Hosni D., Wei J., Abbas G., Ga’al H., Jianchu M. (2018) Artif Cells Nanomed Biotechnol., 46, 558-567.
13. Srinivasan A., Giri A.P. and Gupta V.S. (2006) Cell Mol Biol Lett., 11, 132-154.
14. Sharma H.C., Sharma K.K., Seetharama N. and Ortiz R. (2000) Electron. J. Biotechnol., 3, 76-95.
15. Swathi M., Mishra P.K., Lokya V., Swaroop V., Mallikarjuna N., Dutta Gupta A. (2016) Front. Physiol., 7, 388.
16. Patil C.D., Borase H.P., Suryawanshi R.K., Patil S.V. (2016) Enzym Microb Technol., 92, 18-25.
17. Santhoshkumar T., Rahuman A.A., Bagavan A., Marimuthu S., Jayaseelan C., Kirthi A.V., Kamaraj C., Rajakumar G., Zahir A.A., Elango G., Velayutham K., Iyappan M., Siva C., Karthik L., Bhaskara Rao K.V. (2012) Exp Parasitol., 132, 156-165
18. Rajakumar G., Rahuman A.A. (2011) Acta Trop. 118, 196-203.
19. Kanniah P., Radhamani J., Chelliah P., Muthusamy N., Thangapandi E.J.J.S.B., Thangapandi S., Balakrishnan J.R., Shanmugam R. (2020) Chem.Select., 5, 2322-2331.
20. Adur A.J., Nandini N., Shilpashree M.K., Ramya R., Srinatha N. (2018) J.Photochem. Photobio.B, Bio., 183, 30-34.
21. Kong J.,Yu S. (2007) Acta biochimica et biophysica Sinica. 39, 549-559.
22. Darezereshki E. (2010) Mater. Lett. 64, 1471-1472.
23. Sathyamoorthy R, Mageshwari K. (2012) Physica E. 47, 157-161.
24. Chattopadhyay S, Lo HC, Hsu CH, Chen LC, Chen KH. (2005) Chem.Mater. 17, 553-559.
25. HuangY F, Chang HT, Tan W. (2008) Anal.Chem. 80, 567-572.
26. Chowdhury J., Ghosh M. (2007) J.Colloid. Inter.Sci. 277, 121-127.
27. Ajitha B, Ashok Kumar Reddy Y., Sreedhara Reddy P. (2014) Acta Part A. 128, 257-262.
28. Srinivasan V.P., Nathan S.S., Thanigaivel A., Edwin E.S., Ponsankar A., Selin-Rani S., Pradeepa V. (2016) Chemosphere. 155, 336-347.
29. Zaka S.M., Abbas N., Shad S.A., Shah R.M. (2014) Phytoparasitica, 42, 493-501.
30. Nel A., Xia T., Madler L., Li N. (2006) Science, 311, 622-627.
31. Kramarz P., Kafel A. (2003) Environ. Pollut., 126, 1-3.
32. Stone D., Jepson P., Laskowski R. (2011) Comp. Biochem. Physiol. Part C., 132, 105-112.
33. Bagavan A., Kamaraj C., Elango G., Zahir A.A., Rahuman A.A. (2009) Vet Parasitol., 166(3-4), 286-292.
34. Isman M.B. (2006) Annu Rev Entomol., 51, 46-66
35. Shekari M., Sendi J.J., Etebari K., Zibaee A., Shadparvar A. (2008) Pest Biochem Physiol., 91, 66-74.
36. Zahir A.A., Bagavan A., Kamaraj C., Elango G., Rahuman A.A. (2012) J Biopest., 5, 95-102
37. Santhoshkumar T., Rahuman A.A., Rajakumar G., Marimuthu S., Bagavan A., Jayaseelan C. (2011) Parasitol. Res., 108, 693-702.
38. Ki H.Y., Kim J.H., Kwon S.C., Jeong S.H. (2007) J Mater Sci. 42, 8020-8024.
39. Chakravarthy A.K., Chandrashekharaiah M., Khandakoor S.B., Bhattacharyya A., Dhanabala K., Gurunatha K., Ramesh P. (2012) Curr. Biot., 6, 271-281.
40. Lesniak A., Fenaroli F., Monopoli M.P., Aberg C., Dawson K.A. and Salvati A. (2012) ACS Nano, 6, 5845-5857.
41. Schmutterer H. (1990) Annuval Review of Entomology, 35, 271-297.
42. Kantrao S., Ravindra M.A., Kamala Jayanthi P.D., Venkataraman A. (2014) J. Nanoparticles, 829718.
43. Ernest V., Shiny P.J., Mukherjee A., Chandrasekaran N. (2012) Carbohydr, (352), 60-64.
44. Gole A., Dash C., Ramakrishnan V., Sainkar S.R., Mandale A.B., Rao M., Sastry M. (2001) Langmuir., 17 (5), 1674-1679.