PREVALENCE OF COLISTIN RESISTANCE AMONG NON-FERMENTING GRAM NEGATIVE BACTERIA ISOLATED FROM VARIOUS CLINICAL SPECIMENS IN A TERTIARY CARE HOSPITAL

S.T. SONI¹*, D.G. TADA², S. PRAJAPATI³, M. SINGH⁴, M. BHUT⁵, J. DAIVIK⁶, T. RANGOONWALA^7
1Department of Microbiology, B. J. Medical College, Gujarat University, Ahmedabad, Gujarat, 380016, India
²Department of Microbiology, B. J. Medical College, Gujarat University, Ahmedabad, Gujarat, 380016, India
³Department of Microbiology, B. J. Medical College, Gujarat University, Ahmedabad, Gujarat, 380016, India
⁴Department of Microbiology, B. J. Medical College, Gujarat University, Ahmedabad, Gujarat, 380016, India
⁵Department of Microbiology, B. J. Medical College, Gujarat University, Ahmedabad, Gujarat, 380016, India
⁶Department of Microbiology, B. J. Medical College, Gujarat University, Ahmedabad, Gujarat, 380016, India
⁷Department of Microbiology, B. J. Medical College, Gujarat University, Ahmedabad, Gujarat, 380016, India
* Corresponding Author : drsumeetasoni@gmail.com

Received : 04-09-2021     Accepted : 27-09-2021     Published : 30-09-2021
Volume : 13     Issue : 4       Pages : 1972 - 1974
Int J Microbiol Res 13.4 (2021):1972-1974

Keywords : Colistin, NFGNB, Colistin Screen Agar, Broth Microdilution-BMD
Conflict of Interest : None declared
Acknowledgements/Funding : Authors are thankful to Department of Microbiology, B. J. Medical College, Gujarat University, Ahmedabad, Gujarat, 380016, India
Author Contribution : All authors equally contributed

Background and Objectives: Indiscriminate use of antimicrobials has caused emergence of Multidrug Resistance bacteria (MDR). Resistance to several antibiotics including Colistin is great challenge in therapy. NFGNB being common cause of Hospital Acquired Infections and their resistance to various antimicrobial drugs, significantly influences morbidity, mortality and high cost of treatment of infected patients. This study aims to find out the prevalence of colistin resistance among non-fermenting gram-negative bacteria isolated from various clinical specimens received at Bacteriology Laboratory, Civil Hospital, Ahmedabad. Methods: The study was conducted over a period of 1 year from October 2020 to October 2021. Total 41390 samples received. NFGNB were isolated by standard microbiological identification technique. The isolates were subjected to antimicrobial susceptibility testing by Modified Kirby Bauer Disc Diffusion method. Colistin sensitivity is screened on Colistin Screen Agar (CSA) and resistance is confirmed by BMD (Broth Micro Dilution). Result: Among 41390 samples tested, 17491 had positive culture growth, 7610 isolates had gram-negative bacterial growth, out of which 4393 were non fermenters, in which 53 screened for Colistin resistance. Interpretation and Conclusion: This study provides prevalence of colistin resistance among non-fermenting gram-negative bacteria. P. aeruginosa causing hospital associated infections shows emergence of resistance to commonly used antimicrobials agents and colistin is last resort to treat MDR organisms. CL resistance in MDR organisms is a serious threat and hence there is a need of awareness among clinicians about judicious use of antibiotics and strict infection control practices needs to be followed