MORPHOLOGICAL AND BIOCHEMICAL PROFILES OF YEASTS FROM LEGUMINOUS CROPS

K. ARCHANA¹*, B.C. MALLESHA^2
1Department of Agricultural Microbiology, University of Agricultural Sciences, GKVK, Bengaluru, 560065, Karnataka, India
²Department of Agricultural Microbiology, University of Agricultural Sciences, GKVK, Bengaluru, 560065, Karnataka, India
* Corresponding Author : archanamicro11@gmail.com

Received : 03-01-2021     Accepted : 26-01-2021     Published : 30-01-2021
Volume : 13     Issue : 1       Pages : 1935 - 1938
Int J Microbiol Res 13.1 (2021):1935-1938

Keywords : Yeast isolates, Sugar fermentation, Nitrate reduction, leguminous crop
Academic Editor : Marcos Antonio Pesquero
Conflict of Interest : None declared
Acknowledgements/Funding : Author thankful to Department of Agricultural Microbiology, University of Agricultural Sciences, Bengaluru, 560065, India
Author Contribution : All authors equally contributed

The study was conducted to isolate yeasts from phyllosphere and rhizosphere soil of different leguminous crops from Bengaluru and Dharwad districts. Thirty-five yeast isolates were obtained using four different media. Microscopic observations revealed that most of the yeast isolates were oval and the color of the yeast isolates varied from white, milky white, dull-white, pink and yellow. Nearly 85 percent of the yeast isolates were found to be acid producers and capable of utilizing six different sugars as carbon source. All the yeast isolates showed positive growth for tryptophan and indole production test. Among thirty-five yeast isolates, twenty-five isolates showed positive for starch hydrolysis, seventeen isolates were positive for cellulase production test and six for urease test. About 35-40 percent of the yeast isolates were found to grow fairly in all the supplemented nitrogen sources

1. Khaled, A. E. and Krishnapillai, S. (2006) Mycoscience, 47, 25-35.
2. Avinash C.S. and Patil B.L. (2018) Journal of Pharmacognosy and Phytochemistry, 7(4), 2097-2102.
3. Amprayn K.O., Rose M.T., Kecskés M., Pereg L., Nguyen H.T. and Kennedy I.R. (2012) Applied Soil Ecology, 61, 295-299.
4. Botha A. (2011) Soil Biology and Biochemistry, 43(1), 1-8.
5. Cabrini P.G., Sala F.C. and Magri M.M.R. (2019) Horticultura Brasileira, 37(3), 266-271.
6. Fu S.F., Sun P.F., Lu H.Y., Wei J.Y., Xiao H.S., Fang W.T., Cheng B.Y. and Chou J.Y. (2016) Fungal Biology, 120(3), 433-448.
7. Patel P., Desai H., Krishnamurthy R. and Shah A. (2017) Journal of Pure and Applied Microbiology, 11(3):1549-1557.
8. Swapan K. G. (2011) Agriculture and Biology Journal of North America, 2(8), 1166-1170.
9. Wickerham L.J., Lockwood L.B., Pettijohn O.G. and Ward G.E. (1944) Journal of bacteriology, 48(4), 413.
10. Liu Y.Y., Chen H.W. and Chou J.Y. (2016) PLoS One, 11(8), 0160524.
11. Lyudmila, V. I., Yelena. V. B., Ramza, Z. B. and Togzhan, D. M. (2015) Microbiology Research, 175, 78-83.
12. Prasad P., Singh T. and Bedi S. (2014) International Journal of Current Research and Academic Review, 2, 60-70.
13. Hakim A. S., Azza S. M., Abuelnaga S. M. and Rasha M. H. S. (2013) International Journal of Microbiology Research, 4(1), 95-100.
14. Gordon B. R. and Keith H. L. (1940) Journal of Bacteriology, 39(4), 399-404.
15. Siverio J.M. (2002) Fems Microbiology Reviews, 26(3), 277-284.