PREVALENCE OF LEGIONELLA PNEUMOPHILA AMONG LRTI PATIENTS AND ENVIRONMENTAL WATER SAMPLE FROM A TERTIARY CARE HOSPITAL

C. ANITHA¹*, S. SENTHAMARAI², S. SIVASANKARI³, R. LOKESHWARI^4
1Department of Microbiology, Meenakshi Medical college Hospital and Research Institute, Enathur, Kanchipuram, 631552, India
²Department of Microbiology, Meenakshi Medical college Hospital and Research Institute, Enathur, Kanchipuram, 631552, India
³Department of Microbiology, Meenakshi Medical college Hospital and Research Institute, Enathur, Kanchipuram, 631552, India
⁴Department of Microbiology, Meenakshi Medical college Hospital and Research Institute, Enathur, Kanchipuram, 631552, India
* Corresponding Author : ani.phd@gmail.com

Received : 01-06-2020     Accepted : 27-06-2020     Published : 30-06-2020
Volume : 12     Issue : 6       Pages : 1848 - 1851
Int J Microbiol Res 12.6 (2020):1848-1851

Keywords : Infection, Pathogen, Pneumonia
Academic Editor : Dr Carlos Aa Chagas, Dr Ravindran, Dr Prabhu, Ranabijuli S., Dr Rajesh Kumar Sahu
Conflict of Interest : None declared
Acknowledgements/Funding : Authors are thankful to ICMR for their selection of this project under ICMR STS funding project. Authors are also thankful to Department of Microbiology, Meenakshi Medical college Hospital and Research Institute, Enathur, Kanchipuram, 631552, India
Author Contribution : All authors equally contributed

Legionella an important respiratory pathogen which is often neglected and under reported. It is known to be the second most common cause of Community acquired pneumonia. Hence, the present study was undertaken to know the prevalence of Legionella from hospital environmental water samples and from patients admitted with lower respiratory tract infection. Objective: To know the prevalence of Legionella pneumophila and to study about water as a source of Legionella in tertiary care hospital. Method: Out of 100 samples collected 82 were sputum samples & 9 were Broncho alveolar lavage,5 was pleural fluid. The samples were processed according to standard protocol. 21 Water samples were collected from taps of various ICU’s and wards. The samples were streaked on both legionella agar medium with supplement and BA without supplement and incubated at 37 C at 5 -10 %Co2 for 3- 7 days presence of colonies on legionella agar medium alone were confirmed as legionella . Results: Out of 100 samples collected 96 (96%) samples were included in the study. Of 96 samples 82(85.4%) were sputum samples in which 12 (14.6%) grew legionella in medium with supplement. Out of 21 water samples collected from various wards and ICU‘s only 3 (14.3%) were positive for the growth of legionella. Conclusion:Our study emphasis the need for further studies involving multi centers throughout state. Our study also urges a need to fill the control gaps and Implement surveillance on environmental systems in Hospitals.

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