DIVERSITY OF MICRO-FLORA IN DIFFERENT COMBINATIONS OF PRESS-MUD FOR BIOGAS PRODUCTION

S. PAROHA¹*, A. KACHHWAHA², S. SINGH^3
1National Sugar Institute, Kanpur, 208017, Uttar Pradesh, India
²National Sugar Institute, Kanpur, 208017, Uttar Pradesh, India
³National Sugar Institute, Kanpur, 208017, Uttar Pradesh, India
* Corresponding Author : seemaparoha@rediffmail.com

Received : 06-04-2020     Accepted : 28-04-2020     Published : 30-04-2020
Volume : 12     Issue : 4       Pages : 1821 - 1827
Int J Microbiol Res 12.4 (2020):1821-1827

Keywords : Biogas, Press mud, Bacteria and Fungi
Academic Editor : Dr Ranjana Hawaldar
Conflict of Interest : None declared
Acknowledgements/Funding : Authors are thankful to Biochemistry Division, National Sugar Institute, Kanpur, 208017, Uttar Pradesh, India
Author Contribution : All authors equally contributed

A bench scale model of Lab trials with 10 different combinations of Press-mud and other materials (FYM, sewage, spent wash, Leafy trash and green tops) were taken to find out pattern of distribution of different microorganisms responsible for Biogas production. Bacterial colonies observed in slurry at initial, 7th and 14th day were Rhizobium spp., Azatobactor spp., Bacillus subtilis, Pseudomonas aurigunosa, Klebsilla pneumoniae, E. coli, Vibro cholera, Yarsinia enterocolitica, Micrococcus, Flovobacterium megningosepticum, Clostridium perfringens and Streptococcus lactis. The bacterial strain obtained after 14th day were Methanothrix soehngenii, Clostridium perfringens, Methanobacterium sp., Methanosaricina spp., Methanobrevibacter spp., and Bacillus licheniformis but fungal colony were absent after 14th day of trail. The fungal colonies observed till 14th day were Rhizopus, Penicillium chrysogenum, Aspergillus niger, Aspergillus flavus, Aspergillus fumigatous, Microsporum spp., Rhizocktonia solani, Saccharomyces spp., Fusarium spp. Thus, over all analysis indicated that there were only bacterial strains which play an important role in biogas production in utilizing the industrial waste i.e. press mud with different combination for production of biogas. The best results were seen in 80:20 combination i.e., T1 which was composed of 80% press mud and 20% FYM and observed by maximum filling of biogas in balloon made it bigger in size than the other combinations.

1. Tafdrup S. (1995) Biomass Bioenerg., 9, 1-5, 303-314.
2. Somasegaran and Hoben (1985) University of Hawaii, NIFTAL, Project and Micren, Dept. of Agronomy and Soil, 1300.
3. Josey D.P., Beyhon J.L., Johnson A.W.B., Beringer J.E. (1979) J. Appl. Bact., 46, 343-350.
4. Cappuccino J.G., Sherman N. (1999) “Microbiology? A Laboratory Manual”, (34d edn.). Rockland Community College, Suffern: New York.
5. Gillman J.C. (1957) A manual of soil fungi, Revised 2nd edn., Oxford and I.B.H Publishing company (Indian reprint), Calcutta, Bombay, New Delhi – 250.
6. Wirth R., Kovács E., Maróti G., Bagi Z., Rákhely G., et al. (2012) Biotechnol Biofuels, 5, 41.
7. McGhee T.J. (1968) Water Sewage Works, 115, 162-166.
8. Schlüter A., Bekel T., Diaz N.N., Dondrup M., Eichenlaub R., et al. (2008) J Biotechnol.,136, 77-90.
9. Dahiya J., Singh D., Nigam P. (2001a) Biores. Technol.,78, 111-114.