MOLECULAR SCREENING OF FECB GENE MUTATION THROUGH PCR RFLP METHOD IN DIFFERENT SHEEP BREEDS OF TAMIL NADU

R. SARAVANAN¹*, P. KUMARASAMY², R. ANILKUMAR³, A.K. THIRUVENKADAN⁴, N. MURALI^5
1Department of Animal Genetics and Breeding, Veterinary College and Research Institute, Namakkal, 637002, Tamil Nadu Veterinary and Animal Sciences University, Chennai, 600051, Tamil Nadu, India
²Controller of Examinations, Tamil Nadu Veterinary and Animal Sciences University, Chennai, 600051, Tamil Nadu, India
³Professor and Head, Sheep Breeding Research Station, Sandynallah, Ooty, 643005, The Nilagiris, India
⁴Department of Animal Genetics and Breeding, Veterinary College and Research Institute, Namakkal, 637002, Tamil Nadu Veterinary and Animal Sciences University, Chennai, 600051, Tamil Nadu, India
⁵Department of Animal Genetics and Breeding, Veterinary College and Research Institute, Namakkal, 637002, Tamil Nadu Veterinary and Animal Sciences University, Chennai, 600051, Tamil Nadu, India
* Corresponding Author : mrsagb@gmail.com

Received : 16-03-2020     Accepted : 08-04-2020     Published : 30-04-2020
Volume : 12     Issue : 4       Pages : 725 - 727
Genetics 12.4 (2020):725-727

Keywords : FecB, Fecundity gene, PCR RFLP, Niligiri, Sandyno
Academic Editor : Dr M D Hivre
Conflict of Interest : None declared
Acknowledgements/Funding : Authors are thankful to Principal Investigator, Central Sector Scheme on Biotech Centre for Fecundity Genes and Tamil Nadu Veterinary and Animal Sciences University, Chennai, 600051, Tamil Nadu, India
Author Contribution : All authors equally contributed

The indigenous sheep breeds Tamil Nadu were screened for the presence of fecundity gene: FecB as part of FecB gene introgression program. A total of 864 genomic DNA samples of Coimbatore, Mecheri, Niligiri, Trichy black and synthetic Sandyno sheep breeds were used to screen the FecB gene mutation through PCR RFLP method. The screening results showed that considerable percentage of FecB mutation present in both Nilagiri and Sandyno synthetic sheep breeds. In Nilagiri sheep the frequency of wild, FecB heterozygote (B+) and homozygote (BB) genotype were 54.71, 35.87 and 9.41 percent respectively. In Sandyno sheep the frequency of wild, FecB heterozygote (B+) and homozygote (BB) genotype were 88.14, 11.86 and 0.00 percent respectively. Whereas in Coimbatore, Mecheri and Tiruchy Black sheep FecB gene was absent in all samples screened and all animals showed only the uncut 140 bp band wild type (++) genotype.

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