ISOLATION AND CHARACTERIZATION OF NATIVE POTASH MOBILIZING PLANT GROWTH PROMOTING RHIZOSPHERIC BACTERIA

A.S. PATEL¹*, H.N. SHELAT², R.V. VYAS^3
1Department of Agricultural Microbiology, B A College of Agriculture, Anand Agricultural University, Anand, 388 110, Gujrat, India
²Department of Agricultural Microbiology, B A College of Agriculture, Anand Agricultural University, Anand, 388 110, Gujrat, India
³Department of Agricultural Microbiology, B A College of Agriculture, Anand Agricultural University, Anand, 388 110, Gujrat, India
* Corresponding Author : aspatel1990@yahoo.co.in

Received : 01-03-2020     Accepted : 27-03-2020     Published : 30-03-2020
Volume : 12     Issue : 3       Pages : 1789 - 1793
Int J Microbiol Res 12.3 (2020):1789-1793

Keywords : Rhizosphere, KMB, Potash, Acinetobacter, PGPR
Academic Editor : Dr Carlos Aa Chagas
Conflict of Interest : None declared
Acknowledgements/Funding : Authors are thankful to Department of Agricultural Microbiology, B A College of Agriculture, Anand Agricultural University, Anand, 388 110, Gujrat, India.
Author Contribution : All authors equally contributed

Research was carried out to explore plant growth promoting abilities of native rhizospheric potash mobilizing bacterial (KMB) isolates. Seventeen prominent cultures were selected on the basis of the zone of potash solubilization on Aleksandrov agar supplemented with mica which exhibited organic acid and capsular polysaccharides production. Fourteen isolates were positive for acidic exo-polysaccharides. Five prominent cultures were selected on the basis of PGP traits i.e. solubilization of phosphate and zinc; production of indole acetic acid and siderophore; showing various enzyme activity viz. ACC-deaminase, lipase, protease and cellulase. Moreover, these isolates manage phytopathogenic fungi viz. Fusarium oxysporum, Fusarium solani, Pythium aphanidermatum, Macrophomina phaseolina and Alternaria alternata. Isolates were able to tolerate wide range of pH, temperature and NaCl concentrations. On the basis of morphological, physiological, biochemical and molecular techniques, native KMB PGPR isolates were characterized as; Acinetobacter pittii, A. oleivorans, A. baumannii, A. calcoaceticus and A. junii. The results endow with a basis for understanding the beneficial effects of KMB for deploying the strains and or its consortium in industrial production of bio-fertilizer cum bio-control agents for agricultural crops.

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