ISSN : 0975-5276
EISSN : 0975-9174
S. SINGH1, P.K. GILL2*, T. MISHRA3, H.S. DHALIWAL4
1Department of Biotechnology, Eternal University, Baru Sahib (Sirmour), 173101, Himachal Pradesh, India
2Centre for Interdisciplinary Biomedical Research, Adesh University, Bathinda, 151109, Punjab, India
3Department of Biotechnology, Eternal University, Baru Sahib (Sirmour), 173101, Himachal Pradesh, India
4Department of Biotechnology, Eternal University, Baru Sahib (Sirmour), 173101, Himachal Pradesh, India
* Corresponding Author : pjkgill@gmail.com
Received : 01-01-2020 Accepted : 26-01-2020 Published : 30-01-2020
Volume : 12 Issue : 1 Pages : 1767 - 1770
Int J Microbiol Res 12.1 (2020):1767-1770
Keywords : Biocontrol, Fungal isolates, Host specificity, Parthenium
Academic Editor : Dr S T Rajan, U. Y. Kandekar, Dr Prabhjot Kaur Gill
Conflict of Interest : None declared
Acknowledgements/Funding : Authors are thankful to Centre for Interdisciplinary Biomedical Research, Adesh University, Bathinda, 151109, Punjab, India
Author Contribution : All authors equally contributed
The biological control through various isolated strains of fungi from naturally infected Parthenium plants collected from different locations of Punjab. Total nine fungal species were isolated viz., three Alternaria sp., four Fusarium sp., Cladosporium sp. and Cephalosporium sp. Isolated strain’s pathogenicity was tested individually as well as in combination (both in lab and field conditions) against Parthenium with different control plants. Out of all the isolated strains, Fusarium equiseti followed by Fusarium solani and Cephalosporium sp. showed defoliation of leaves in laboratory conditions whereas less effective in the field conditions. In another approach, disc plate technique was used to investigate the spore germination time and fungus penetration route with a Scanning Electron Microscope. In addition, methods for fungal inoculum development with silica beads, wheat bran, CM-cellulose and Tween80 were also explored for spraying of fungal cultures in the field.
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