ENDOPHYTIC COLONIZATION AND GROWTH PROMOTION OF CAULIFLOWER PLANT BY Bacillus thuringiensis

S.S. THILAGAVATHI¹, G. PRASAD²*
¹Department of Agricultural Microbiology, Tamil Nadu Agricultural University, Coimbatore, 641003, Tamil Nadu, India
²Department of Agricultural Microbiology, Tamil Nadu Agricultural University, Coimbatore, 641003, Tamil Nadu, India
* Corresponding Author : prasadg@tnau.ac.in

Received : 03-01-2020     Accepted : 26-01-2020     Published : 30-01-2020
Volume : 12     Issue : 1       Pages : 1771 - 1775
Int J Microbiol Res 12.1 (2020):1771-1775

Keywords : Bacillus thuringiensis, Cauliflower, PGPR
Academic Editor : Abdel Raheem M. A., U. Y. Kandekar, Rajpal Diwakar, Marcos Antonio Pesquero, Dr Prabhjot Kaur Gill
Conflict of Interest : None declared
Acknowledgements/Funding : Authors are thankful to Department of Agricultural Microbiology, Tamil Nadu Agricultural University, Coimbatore, 641003, Tamil Nadu, India
Author Contribution : All authors equally contributed

Bacterial endophytes are endosymbiotic microorganisms live inside plants for at least part of their life cycle without causing visible symptoms and involved in plant growth promotion. The present investigation the endophytic B. thuringiensis was isolated from the plant sample. Five isolates were confirmed on the basis of crystal formation and identified as Bacillus thuringiensis. The colonization capacity of the isolates was studied by recovering B. thuringiensis from the inoculated leaf and stem (2.9 × 107/g) and root (3.9 × 107/g) of the plants. The cauliflower seed germination and seedling development, seven days after planting showed significant increase over un-inoculated control. Further, the selected isolates were screened for the production of cell wall degrading enzymes and multiple plant growth promoting (PGP) traits viz. phosphate (P) and zinc (Zn) solubilization abilities. All five endophytic B. thuringiensis isolates were found positive for the production of cell wall degrading enzymes viz., cellulase, pectinase, protease, endoglucanase, and also having capacity to solubilize phosphate and zinc. The maximum phosphate and Zn solubilization was achieved by the standard strain HD1 followed by isolate CF4 followed by CF2. The ability of Bacillus thuringiensis isolates for IAA production, Gibberellic acid production and Siderophore production too was assessed. IAA and GA3 production were found to be maximum with CF4 (42.67 and 356.8 ?g l-1 respectively), followed by CF2 (31.51 and 296 ?g l-1). Maximum siderophore production was observed in CF4 (374 mg l-1) followed by CF2 (270 mg l-1).

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