COMPARATIVE STUDY OF SPUTUM AFB AND CBNAAT IN SUSPECTED TB PATIENTS WITHOUT HIV ATTENDING A TERTIARY CARE HOSPITAL OF WESTERN ODISHA

S. MISHRA¹, S. MUKHERJEE²*, A. DASH³, S. TIWARI^4
1Department of Microbiology, Hitech Medical College, Rourkela,769004, Odisha, India
²Department of Microbiology, Hitech Medical College, Rourkela,769004, Odisha, India
³Department of Microbiology, Hitech Medical College, Rourkela,769004, Odisha, India
⁴Department of Microbiology, Hitech Medical College, Rourkela,769004, Odisha, India
* Corresponding Author : shuvankar.mukherjee963@gmail.com

Received : 06-01-2020     Accepted : 26-01-2020     Published : 30-01-2020
Volume : 12     Issue : 1       Pages : 1764 - 1766
Int J Microbiol Res 12.1 (2020):1764-1766

Keywords : TB, Sputum, AFB, CBNAAT
Academic Editor : Kiranjot Kaur
Conflict of Interest : None declared
Acknowledgements/Funding : Authors are thankful to Department of Microbiology, Hitech Medical College, Rourkela,769004, Odisha, India
Author Contribution : All authors equally contributed

Background: Tuberculosis is a major health problem of India. Sputum AFB smear study is the age -old method of laboratory diagnosis of Pulmonary tuberculosis. CB NAAT is a recent nucleic acid based technology to diagnose Tuberculosis. Although it is highly sensitive and specific for diagnosis of TB it is costly and not available in resource poor area. We have conducted a research to compare efficacy of sputum AFB and CB NAAT to diagnose TB in suspected HIV negative patient attending a tertiary care hospital of Western Odisha. Methods: Sputum sample of 823 suspected TB patient who were HIV negative were collected and subjected to both AFB smear study and CB NAAT. Result: Among 823 samples 61(7.4%) were positive for AFB and 65(7.89%) were positive by CB NAAT.1 patient positive by AFB was negative by CB NAAT. And 4 patients who were negative for AFB were positive by CB NAAT. Conclusion: Sputum AFB is a good screening method for Pulmonary tuberculosis in HIV negative individual.

1. Indian Council of Medical Research (1959) ICMR special report series No. 34. New Delhi.
2. Chadha V.K. (2011) Text book of pulmonary and critical care medicine- 1st ed, Jaypee Brothers Medical Publishers. New Delhi, 489-510.
3. Khatri G.R., Frieden T.R. (2000) Int J Tuberc Lung Dis., 4, 193-200.
4. Christopher J.L.M., Katrina F.O., Caterina G., Stephen S.L., Timothy M.W., D Allen R., et al., (2013) The Lancet, 384(9947), 1005-1070.
5. Gupta H., Pandey R.P., Prajapati M.K., et al., (2019) J. Evolution Med. Dent. Sci., 8(50), 3764-3768.
6. Pandey P., Pant N.D., Rijal K.R., et al., (2017) PLoS One, 12(1).
7. India TB. Report (2018) New Delhi, Central TB Division, Directorate General of Health Services, Ministry of Health and Family Welfare.
8. Gupta H., Pandey R.P., Prajapati M.K., et al., (2019) J. Evolution Med. Dent. Sci., 8(50), 3764-3768.
9. Khatawkar A.V., Talikoti N., Malkiwadeyar P.K., et al., (2019) J. Evid. Based Med. Healthc., 6(19), 1464-1469.
10. Rao D.P., Sowjanya K.L. (2016) Journal of Evidence Based Medicine and Healthcare, 3(38), 1896-8.
11. Gupta P., Rawat J., Sindhwani G., et al., (2006) Indian J Tuberc., 53, 96-100.
12. Dewan R., Anuradha S., Khanna A., et al., (2015) J Indian Acad Clin Med., 16(2),114-7.