ISSN : 0975-5276
EISSN : 0975-9174
K.G. ANITHA1*, V. GOMATHY2, K. SIVASUBRAMANIAM3
1Department of Crop Management, Agricultural College and Research Institute, Kudumiyanmalai, 622 104, Tamil Nadu Agricultural University, Coimbatore, 641003, India
2Department of Agricultural Microbiology, Agricultural College and Research Institute, Tamil Nadu Agricultural University, Coimbatore, 641003, Tamil Nadu, India
3Department of Crop Management, Agricultural College and Research Institute, Kudumiyanmalai, 622 104, Tamil Nadu Agricultural University, Coimbatore, 641003, India
* Corresponding Author : anithakg@tnau.ac.in
Received : 04-12-2019 Accepted : 26-12-2019 Published : 30-12-2019
Volume : 11 Issue : 12 Pages : 1750 - 1752
Int J Microbiol Res 11.12 (2019):1750-1752
Keywords : Seaweed, Phosphate Solubilising Bacteria, Zinc Solubilising Bacteria, Liquid bioinoculant, Bhendi, Germination
Academic Editor : Dr Ranjana Hawaldar, Kiranjot Kaur, M Azzazi, Dr Sharada T. Rajan
Conflict of Interest : None declared
Acknowledgements/Funding : Authors are thankful to Department of Crop Management, Agricultural College and Research Institute, Kudumiyanmalai, 622 104, Tamil Nadu Agricultural University, Coimbatore, 641003, India. Authors are also thankful to Department of Agricultural Microbiology, Agricultural College and Research Institute, Tamil Nadu Agricultural University, Coimbatore, 641003, India
Author Contribution : All authors equally contributed
The GC-MS studies revealed the presence of antifungal bioactive compounds and antioxidant compounds especially phenols in the seaweed extracts of Gracilaria cervicornis and G. gracilis. Hence the extract of these two were utilized for the liquid bioinoculant production in combination with the 3 compatible Phosphate Solubilising Bacterial cultures (Pd 1, Pd3) and Zinc Solubilising Bacterial cultures (Zd1). As the microbial load of these bacterial isolates were not affected by the addition of seaweed extract upto 5% level, the final liquid bioinoculant was prepared by growing Pd 1, Pd3 and Zd1 cultures in liquid broth containing 5% seaweed extract. The influence of the prepared ‘seaweed based liquid bioinoculant’ on the germination of bhendi was observed for the bhendi crop (Hybrid: Green Gold Plus) by imposing 5 treatments and one control. The treatments imposed were T1 - Azospirillum + PB +KRB, T2- PSB + ZSB, T3- PSB + ZSB + 1 % Seaweed Extract, T4- PSB + ZSB + 3 % Seaweed Extract, T5- PSB + ZSB + 5 % Seaweed Extract and Control. T2 (78.9%) recorded a germination percent lesser than T1 (80.3%) which is the existing recommendation of bioinoculants. T3 (83.5%) and T4 (83.7) were statistically on par. Germination % observed in T5 was statistically significant than all other treatments. The % increase over control showed that influence of T2 treatment was lesser than the existing recommended bioinoculants treatment (T1). But the newly formed ‘seaweed based liquid bioinoculant’ at 1%, 3% and 5% increased the germination percent. T5 treatment recorded 15.27 % increase over control. When compared the % increase over T1 (existing bioinoculant recommendation) it was 6.23, 4.23 and 4.0 for T5, T4 and T3 respectively. This study proved that this newly formulated seaweed based liquid bioinocuant enhances germination of bhendi crop.
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