BIOSURFACTANT PRODUCTION FROM CRUDE OIL SOIL BACTERIUM

D.J. YADAV¹, M.M. VAIDYA², H.A. PATHAN³*, S.D. WAVHAL^4
1Department of Biotechnology, Ismail Yusuf College, Mumbai, 400060, University of Mumbai, Mumbai, Maharashtra, India
²Department of Biotechnology, Ismail Yusuf College, Mumbai, 400060, University of Mumbai, Mumbai, Maharashtra, India
³Department of Biotechnology, Ismail Yusuf College, Mumbai, 400060, University of Mumbai, Mumbai, Maharashtra, India
⁴Department of Biotechnology, Ismail Yusuf College, Mumbai, 400060, University of Mumbai, Mumbai, Maharashtra, India
* Corresponding Author : hinaa_123@yahoo.com

Received : 15-08-2019     Accepted : 26-09-2019     Published : 30-09-2019
Volume : 11     Issue : 9       Pages : 1705 - 1707
Int J Microbiol Res 11.9 (2019):1705-1707

Keywords : Biosurfactant, waste engine oil, Lysinibacillus
Conflict of Interest : None declared
Acknowledgements/Funding : Authors are thankful to Department of Biotechnology, Ismail Yusuf College, Mumbai, 400060, University of Mumbai, Mumbai, Maharashtra, India. Authors are also thankful to National Collection of Industrial Microorganisms (NCIM), Pune for Sequencing
Author Contribution : All authors equally contributed

The main criteria of this research were to produce Biosurfactant by bacteria isolated from the soil sample contaminated with crude oil. To carry out this work we have isolated organism from the garage soil (crude oil spill). This strain was subjected to screening test (Bath Assay) for biosurfactant production. The isolated strain was characterised by 16s RNA sequencing (NCIM-CSIR-NCL) found to be resembling Lysinibacillus tabacifolii strain K3514. Crude oil used was waste engine oil as a source of carbon for Biosurfactant production. Biosurfactant are extracellular surface-active compounds produced by bacteria, fungi and yeast. Most microbial surfactant are complex molecules, comprising different structures that include lipopolypeptide, glycolipid, polysaccharide protein complexes, fatty acids and phospholipids. Fermenter production of biosurfactant was carried out in MSM and in Whey broth with waste crude oil from garage as a source of carbon. Biosurfactant was extracted and purified in solvent system Ethyl acetate: methanol (4:1). The biosurfactant produced was dissolved in chloroform and separated on TLC (thin layer chromatography). The separated spots were characterised by GC- MS (Gas chromatography-mass spectrophotometer).

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