STUDY ON VIRULENCE FACTORS & ANTIMICROBIAL RESISTANCE AMONG ENTEROCOCCI SP., ISOLATED FROM VARIOUS CLINICAL SAMPLES IN A TERTIARY CARE HOSPITAL, KANCHIPURAM, INDIA

V.M. Somasunder¹, M. Kamalraj²*, S. Senthamarai³, S. Sivasankari⁴, C. Anitha⁵, K. Akila^6
1Department of Microbiology, Meenakshi Medical College Hospital & Research Institute, Kanchipuram, 631552, MAHER University, Chennai, 600078, Tamil Nadu, India
²Department of Microbiology, Sri Muthukumaran Medical College, Hospital & Research Institute, Chennai, 600069, The Tamil Nadu Dr. M.G.R. Medical University, Chennai, 600032, Tamil Nadu, India
³Department of Microbiology, Meenakshi Medical College Hospital & Research Institute, Kanchipuram, 631552, MAHER University, Chennai, 600078, Tamil Nadu, India
⁴Department of Microbiology, Meenakshi Medical College Hospital & Research Institute, Kanchipuram, 631552, MAHER University, Chennai, 600078, Tamil Nadu, India
⁵Department of Microbiology, Meenakshi Medical College Hospital & Research Institute, Kanchipuram, 631552, MAHER University, Chennai, 600078, Tamil Nadu, India
⁶Department of Microbiology, Meenakshi Medical College Hospital & Research Institute, Kanchipuram, 631552, MAHER University, Chennai, 600078, Tamil Nadu, India
* Corresponding Author : Kamalraj2019@gmail.com

Received : 10-07-2019     Accepted : 26-07-2019     Published : 30-07-2019
Volume : 11     Issue : 7       Pages : 1670 - 1672
Int J Microbiol Res 11.7 (2019):1670-1672

Keywords : Enterococci, Haemolysin, Gelatinase production
Academic Editor : Dr Ranjana Hawaldar, Sharanya K, Banu S. Geetha, Janagond Anand B
Conflict of Interest : None declared
Acknowledgements/Funding : Authors are thankful to Meenakshi Medical College Hospital & Research Institute, Kanchipuram, Chennai, 631552, MAHER University, Chennai, 600078, Tamil Nadu, India
Author Contribution : All authors equally contributed

Enterococcus previously considered as a normal commensal of gut, is developing fast as a pathogen causing serious and life threatening nosocomial infections. This study was undertaken to determine the prevalence factors & their association with antimicrobial resistance. A total of 56 clinical samples were isolated from a tertiary care hospital. All these samples were identified as per standard conventional methods and detected for the production of virulence factors such as Haemolysin, Gelatinase, & Biofilm production. Antimicrobial susceptibility testing was carried out by disc diffusion method according to the CLSI guidelines. Among 56 isolates, E. faecalis were 43(76.7%), & E. faecium were 13(23.3%), The highest percentage of antibiotic resistance was seen in Erythromycin (83.7% in E. faecalis and 84.7% in E. faecium) followed by Ciprofloxacin (76.7% in E. faecalis and 77% in E. faecium), Tetracycline (74.4% in E. faecalis and 46% in E. faecium) and Ampicillin (32.5% in E. faecalis and 84.7% in E. faecium). In this study, overall 30.2% of E. faecalis & 23.1% of E. faecium showed biofilm production, haemolysin production were 46.5% of E. faecalis and 15.3% of E. faecium, followed by Gelatinase production were 21% of E. faecalis and 15.3% of E. faecium. Though the prevalence of vancomycin resistant Enterococci (VRE) is comparatively high in our study, hence regular monitoring of vancomycin resistance is very crucial for early finding, treatment, application of preventive and control measures.

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