PRODUCTION OF ENZYMES BY ASPERGILLUS NIGER BY USING SURFACE AND SUBMERGED FERMENTATION USING WASTE AGRO-INDUSTRIAL SUBSTRATE

M.A.W. MULLA¹, B. SHAIKH², H.A. PATHAN³*, S.D. WAVHAL^4
1Department of Biotechnology, Ismail Yusuf College, Mumbai, 400060, University of Mumbai, Mumbai, Maharashtra, India
²Department of Biotechnology, Ismail Yusuf College, Mumbai, 400060, University of Mumbai, Mumbai, Maharashtra, India
³Department of Biotechnology, Ismail Yusuf College, Mumbai, 400060, University of Mumbai, Mumbai, Maharashtra, India
⁴Department of Biotechnology, Ismail Yusuf College, Mumbai, 400060, University of Mumbai, Mumbai, Maharashtra, India
* Corresponding Author : hinaa_123@yahoo.com

Received : 25-06-2019     Accepted : 26-07-2019     Published : 30-07-2019
Volume : 11     Issue : 7       Pages : 1638 - 1640
Int J Microbiol Res 11.7 (2019):1638-1640

Keywords : Aspergillus niger, Czapek dox medium, Beta-galactosidase, Beta-fructofuranosidase and keratinase
Conflict of Interest : None declared
Acknowledgements/Funding : Authors are thankful to Ismail Yusuf College, Mumbai, 400060, University of Mumbai, Mumbai, Maharashtra, India
Author Contribution : All author equally contributed

The aim of this project is to carryout to improve the production of enzyme by Aspergillus niger. The effect of alternative carbon source in czapek-dox medium as inducer of beta-galactosidase, beta-fructofuranosidase and keratinase activity. Aspergillus niger was grown in czapek Dox medium with orange peel as inducer for enzyme and czapek medium with human hair and chick feather as a substrate for keratinase production. The organism was grown in surface and submerged condition. The enzymes were extracted and purified and activity was determined by spectrophotometer analysis for beta-galactosidase, beta-fructofuranosidase and keratinase. Thus the alternative carbon source as orange peel, human hair and chick feather exhibit great potential as inducer for the production of generating significant amount of beta-galactosidase , beta-fructofuranosidase and keratinase using waste agro industrial substrate.

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