STUDY ON PREVALENCE OF HEPATITIS C VIRUS INFECTION AND ITS GENOTYPE DISTRIBUTION AMONGST CHRONIC RENAL FAILURE PATIENTS ON MAINTENANCE HEMODIALYSIS: A SINGLE CENTER EXPERIENCE

M.H. PATEL¹, S. SONI²*, A.V. VANIKAR³, P.K. SHAH⁴, H. PATEL^5
1Department of Pathology, Laboratory Medicine, Transfusion Services and Immunohematology, G.R. Doshi and K.M. Mehta Institute of Kidney Diseases & Research Centre (IKDRC)- Dr. H.L. Trivedi Institute of Transplantation Sciences (ITS), Gujarat University, Na
²Department of Microbiology, B.J. Medical College, Ahmedabad, 380016, Gujarat University, Navrangpura, Ahmedabad, 380009, Gujarat, India
³Department of Cell Therapy and Regenerative Medicine, Gujarat University, Navrangpura, Ahmedabad, 380009, Gujarat, India
⁴Department of Microbiology, B.J. Medical College, Ahmedabad, 380016, Gujarat University, Navrangpura, Ahmedabad, 380009, Gujarat, India
⁵Department of Nephrology and Transplantation Medicine, G.R. Doshi and K.M. Mehta Institute of Kidney Diseases & Research Centre (IKDRC)- Dr. H.L. Trivedi Institute of Transplantation Sciences (ITS), Gujarat University, Navrangpura, Ahmedabad, 380009, Guja
* Corresponding Author : drsumeetasoni@gmail.com

Received : 27-06-2019     Accepted : 26-07-2019     Published : 30-07-2019
Volume : 11     Issue : 7       Pages : 1632 - 1634
Int J Microbiol Res 11.7 (2019):1632-1634

Keywords : Hepatitis C Virus, Genotypes, HCV Subtypes, Nested Reverse Transcriptase PCR, Chronic Renal Failure, Haemodialysis
Academic Editor : Dr Dibyajyoti Talukda
Conflict of Interest : None declared
Acknowledgements/Funding : Authors are thankful to Department of Pathology, Laboratory Medicine, Transfusion Services and Immunohematology, G.R. Doshi and K.M. Mehta Institute of Kidney Diseases & Research Centre (IKDRC)- Dr. H.L. Trivedi Institute of Transplantation Sciences (ITS), Gujarat University, Navrangpura, Ahmedabad, 380009, Gujarat, India
Author Contribution : All authors equally contributed

Background: Hepatitis c virus (HCV) infection is a very common infection in chronic renal failure (CRF) patients on maintenance hemodialysis (MHD). Genotype detection is crucial for management of chronic hepatitis c patients, prediction of prognosis, epidemiological study and also for vaccine preparation. Based on the sequence divergence, till date HCV strains are divided into 7 main genotypes and multiple subtypes (67 confirmed, 20 provisional). The study was aimed to find out single centre prevalence and distribution of HCV genotypes in CRF patients on MHD. Methods: Genotyping was performed by nested reverse transcriptase PCR. Isolation of HCV RNA, reverse transcription and nucleic acid amplification of 5’ UR was carried out. Biotinylated oligonucleotide primers were used to generate amplified product and reversely hybridized to type-specific probes on nitro-cellulose strips. Conjugate and substrate were added post-hybridization to observe generated bands which were then matched with control bands. The genotypes studied were 1a to1c, 2a to 2d, 3a to 3f, 4a to 4k, 5a and 6a. Results: Out of 2550, 210 patients (12.14%) (Male: 166, Females: 44) were HCV positive. Genotype 1 was found in 179 (85.2%) and genotype 3 in 31(14.8%) patients. Amongst, genotype 1, subtype 1a, 1b, 1ba and undetermined comprised 71.4%, 24.6%, 0.6% and 3.4% respectively. Amongst, genotype 3, subtypes 3a, 3b and undetermined comprised 70.9%, 9.7% and 19.4% respectively. No other genotypes were found. Conclusion: HCV infection was found in 12.14 % CRF patients on MHD with genotype 1 (85.2%) being predominant followed by genotype 3 (14.8%) in our study.

1. Umar M., Hamama-tul-Bushra, Ahmad M., Khurram M., Usman S., Arif M., Adam T., Minhas Z., Arif A., Naeem A., Ejaz K., Butt Z., Bilal M. (2010) HepatMon 10(3), 205-214.
2. Fabrizi F., Poordad F. F., and Martin P. (2002) Hepatology 36, 3–10.
3. Reddy A. K., Murthy K.D., Lakshmi V. (2005) Indian J Med Microbiol 23, 106-110.
4. KabakçıAlagöz G., Karataylı S.C., Karataylı E., Celik E., Keskin O., Dinç B., et al (2014) Turk J Gastroenterol 25, 405-410.
5. Kartashev V., Döring M., Nieto L., Coletta E., Kaiser R., Sierra S., et al (2016) J ClinVirol 8, 182-189.
6. Hijikata M., Kato N., Ootsuyama Y., Nakagawa M., Shimotohno K. (1991) Proc Natl AcadSci U S A 88, 5547-5551.
7. Ausumal F.M. et.al (1990) Current Protocols in Molecular Biology.Vol.2, Greene Publishing Assoc. and Willey-Interscience, New York, A.1.5
8. Salunkhe P.N., Naik S.R., Semwal S.N., Naik S., Kher V. (1992) Indian J Gastroenterol 11, 16.
9. Chadha M.S., Arankalle V.A., Jha J., Banerjee K. (1993) Vox Sang 64, 127-518.
10. Sumathi S., Valliammai T., Thyagarajan S.P., Malathy S., Madanagopalan N., Sankarnarayan V., et al (1993) Indian J Med Microbiol 11, 291-297.
11. Agarwal S. K., Dash S. C. and Irshad M. (1999) J. Assoc. Physicians India 47, 1139–1143.
12. Jaiswal S.P., Chitnis D.S., Salgia P., Sepaha A., Pandit C.S. (2002) Dialys Transplant 31, 234-238.
13. Yabaji P.M., Shankarkumar A., Shukla A., Bhatia S. (2018) Indian J Med Microbiol 36, 352-356.
14. Frank C., Mohamed M.K., Strickland G.T., Lavanchy D., Arthur R.R., Magder L.S. et al (2000) Lancet 355, 887-891.
15. Mukhopadhyaya A. (2008) J Biosci 33, 465–473.
16. Das B.R., Kundu B., Khandapkar R., Sahni S. (2002) Indian J PatholMicrobiol 45, 323-328.
17. Perez R.M., Ferraz M.L., Figueiredo M.S., Contado D., Koide S., Ferreira A.P., CendorogloNeto M., Medina Pestana J.O., Silva A.E. (2003) J Med Virol 69, 489–494.
18. Marinaki S., Boletis J.N., Sakellariou S., Delladetsima I.K. (2015) World J Hepatol ., 7, 548-558.