HALOPHILIC ABILITIES OF BACTERIA IN SELECTED LOCATIONS OF TAMIL NADU

N. BHARATH KUMAR¹, N.O. GOPAL²*, R. ANANDHAM³, P. JEYAKUMAR^4
1Department of Agricultural Microbiology, Tamil Nadu Agricultural University, Coimbatore, 641003, Tamil Nadu, India
²Department of Agricultural Microbiology, Tamil Nadu Agricultural University, Coimbatore, 641003, Tamil Nadu, India
³Department of Agricultural Microbiology, Tamil Nadu Agricultural University, Coimbatore, 641003, Tamil Nadu, India
⁴Department of Crop Physiology, Tamil Nadu Agricultural University, Coimbatore, 641003, Tamil Nadu, India
* Corresponding Author : nogopal1964@yahoo.com

Received : 01-05-2019     Accepted : 26-05-2019     Published : 30-05-2019
Volume : 11     Issue : 5       Pages : 1569 - 1572
Int J Microbiol Res 11.5 (2019):1569-1572

Keywords : Halophiles, Tindivanam, NaCl, Morphological, Biochemical
Conflict of Interest : None declared
Acknowledgements/Funding : Authors are thankful to Department of Agricultural Microbiology, Tamil Nadu Agricultural University, Coimbatore, 641003, India
Author Contribution : All authors equally contributed

Halophiles are salt loving organisms, which can grow in extreme environments of differential salt concentrations. They extend from prokaryote, archae to eukaryotic microorganisms including algae. Halophiles are classified based on their halotolerance abilities into slight, moderate and extreme halophiles. In this present study, the isolation of halophiles was carried out by enrichment culture method using halophilic medium Tryptic Soya Agar (TSA) as a selective media. Screening of extreme halophiles was done by increasing the NaCl concentration up to 4.31 M. Morphological observations and biochemical tests were performed. Out of 90 isolates, 13 isolates were extreme halophiles and survived up to 4.31 M NaCl and remaining 77 isolates were moderate halophiles.

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