AN INSIGHT INTO THE POTENTIAL MACROFUNGAL LACCASE PRODUCERS OF NORTH EAST, INDIA

T. BORGOHAIN¹, D.J. HAZARIKA², T. GAUTOM³, A. PARVEEN⁴, M. KAKOTI⁵, S. DULLAH⁶, M. BAROOAH⁷, R.C. BORO⁸*
¹Department of Agricultural Biotechnology, Assam Agricultural University, Jorhat, 785013, India
²Department of Agricultural Biotechnology, Assam Agricultural University, Jorhat, 785013, India
³Department of Agricultural Biotechnology, Assam Agricultural University, Jorhat, 785013, India
⁴Department of Agricultural Biotechnology, Assam Agricultural University, Jorhat, 785013, India
⁵Department of Agricultural Biotechnology, Assam Agricultural University, Jorhat, 785013, India
⁶Department of Agricultural Biotechnology, Assam Agricultural University, Jorhat, 785013, India
⁷Department of Agricultural Biotechnology, Assam Agricultural University, Jorhat, 785013, India
⁸Department of Agricultural Biotechnology, Assam Agricultural University, Jorhat, 785013, India
* Corresponding Author : robinboro@gmail.com

Received : 04-04-2019     Accepted : 27-04-2019     Published : 30-04-2019
Volume : 11     Issue : 4       Pages : 1550 - 1556
Int J Microbiol Res 11.4 (2019):1550-1556

Keywords : ABTS, Ganoderma lucidum, Guaiacol, Laccase, Macrofungi, Panus lecomtei, Trametes versicolor, Zymogram
Conflict of Interest : None declared
Acknowledgements/Funding : Authors are thankful to acknowledge the Science and Engineering Research Board (SERB), Department of Science and Technology, Govt. of India for providing fund for conducting the research work. Authors are grateful to the Department of Agricultural Biotechnology and DBT- AAU Centre, Assam Agricultural University for rendering the laboratory space and instrumentation facilities for carrying out the study
Author Contribution : RCB conceived the idea; TB, DJH and RCB designed the study; MK, TG, SD performed DNA isolation and identification; TB, SD performed the laccase activity assay; TB, AP, DJH performed the partial purification of laccase, Native –PAGE analysis; MB, RCB sup

Macrofungal laccases are versatile group of metalloenzymes that oxidize both phenolic and non-phenolic compounds with simultaneous four-electron reduction of molecular oxygen to water. The practical applications of laccases have led to a search for sources of the enzyme from macrofungi of North Eastern (NE) region, India. In this study, thirty previously collected macrofungal strains were screened for laccase activity, of which only nine strains tested laccase positive. Detectable amount of laccase production was observed in all the nine macrofungal strains from 2nd day till 10th day of inoculation. Among the nine strains, it was observed that Trametes versicolor (1), Ganoderma lucidum and Trametes coccinea produced maximum enzyme on 6th day; Trametes versicolor (2), Pleurotus pulmonarius, Leiotrametes lactinea and Panus lecomtei on 8th day whereas Trametes cubensis and Trametes sanguinea showed maximal production on 10th day after inoculation. The partially purified macrofungal laccases exhibited pH optima in acidic pH range (pH 2.5 - 4.5) and were able to resist high temperature ranging from 50 ºC to 70ºC. Zymogram analysis showed significant differences typifying the diversity of macrofungal laccases. All the nine macrofungal strains were capable of oxidizing different ortho- substituted aromatic substrates to varying degrees. Among the nine strains tested, a remarkably high laccase activity was observed by Panus lecomtei (7511 U/L) when ABTS was used as asubstrate.

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