PHARMACOGNOSTICALLY STANDARDISED EXTRACT OF BUTEA MONOSPERMA EXHIBITED ANTIMITOTIC AND ANTICANCER ACTIVITY ON HepG2 CELL LINES

S. SANDHYA¹*, VENKATESWARA RAO², P. VENKANNA^3
1Department of Pharmacognosy, St. James College of Pharmaceutical Sciences, Kerala University of Health Sciences, Chalakudy, Thrissur, 680307, Kerala, India
²Department of Pharmacognosy, School of Pharmacy, Anurag Group of Institutions, Ghatkesar, 501301, Jawaharlal Nehru Technological University, Kakinada, 533003
³Department of Pharmacognosy, MAK college of Pharmacy, Chilkoor, 500075, Jawaharlal Nehru Technological University, Kakinada, 533003, India
* Corresponding Author : sanpharm@gmail.com

Received : 06-03-2019     Accepted : 27-03-2019     Published : 30-03-2019
Volume : 11     Issue : 3       Pages : 1521 - 1527
Int J Microbiol Res 11.3 (2019):1521-1527

Keywords : Butea Monosperma, EAC and DLA Cell Lines, HepG2, Chickpea, Mitotic, Tablets
Conflict of Interest : None declared
Acknowledgements/Funding : Authors are thankful to St. James College of Pharmaceutical Sciences, Kerala University of Health Sciences, Chalakudy, Thrissur, 680307, Kerala, India. The authors express their deep sense of gratitude to Dr Vinod K.R., Principal, Sanjo College of Pharmaceutical Studies, Vellapara, Palakkad, 678702, Kerala. Authors are also thankful to Nalanda College of Pharmacy, Nalgonda, Telangana for supporting this work
Author Contribution : All author equally contributed

Butea monosperma (Fabaceae) is popularly known as 'palas' or ‘Flame of forest’ is traditionally reported to possess aphrodisiac, antidiabetic, antidiarrhoeal, antiestrogenic, antifertility, antistress, chemo preventive, etc. The current work was envisaged on development of a suitable dosage form by incorporating methanolic extract B.monosperma for anti-cancer activity. In microscopic studies it had revealed the presence of abundant xylem fibres and xylem vessels. The methanolic extract of stem bark showed the presence of phytoconstituents like flavonoids, tannins and glycosides. The stem bark extracts was screened for the in vitro anti-cancer activity with different methods like anti mitotic activity, MTT assay and Trypan blue exclusion methods. The methanolic extract of stem bark showed moderate anti-cancer activity compared to standard drugs. Further the extract was formulated into a tablet and it was evaluated. The tablets passed all the evaluation parameters as per standard book of reference.

1. Baruchin A.M., Sagi A., Yoffe B., Ronen M. (2001) Burns, 27(7), 781.
2. PravinaWanjari, Deogade Meena, Daf Sunil (2016) International Ayurvedic Medical Journal,1(1),101-106
3. Kokate C.K. (2008) Practical Pharmacognosy, 123-125.
4. Kailasnath S.T., Rama Krishna K.N., Sai Siva Ramakrishna V., Gourinath A., Satyanarayana Reddy (2004) Intermediate First year Botany, 45-48.
5. Madhavan V., Hema B., Guru Deva M.R., Yoganarsimhan (2009) Indian Journal of Traditional Knowledge, 8 (3), 326-333.
6. Kokate C.K., Purohit A.P., Gokhale S.B. (2006) Pharmacognosy, 1.2-1.4.
7. Wagner H., Bladt S. (2004) Plant drug analysis. A thin layer chromatography atlas, 196-197.
8. Daniel Zips, Howard D Thames, Michael Baumann (2005) In vivo, 19, 1-8.
9. Ch Wong., Kl Goh. (2006) Biomedical Imaging and Intervention Journal, 2(3), e7.
10. Leon Lachman, Herbert A Liberman. (2009)The theory and Practice of Industrial Pharmacy, 293-345.
11. Jain R., Jain S.K. (2010) International Journal of Phytomedicine, 2,186-196.
12. Rahman N., Mahmood R., Rahman H., Haris M. (2014) International Journal of Pharmacy and Pharmaceutical Sciences, 6(8), 173-9.
13. Margret C., Jayakar B. (2010) International Journal of Pharmaceutical Sciences Review and Research, 3(1), 101-110.
14. Rakesh Sharma (2010) The Open nutraceuticals Journal, 3, 129-140.
15. Ghiware N.B., Gattani S.G., Chalikwar S.S. (2010) International Journal of Pharmtech Research, 2(1),171-176.
16. Mishra U.S., Murthy P.N., Sahoo S.K., Sahu K.C. (2012) International Journal of Pharmacy, 2(1), 181-186.
17. Gupta S., Gahlot K. (2012) Asian journal of pharmaceutical and clinical Research, 5(1), 148-153.
18. Desai P.P., Patel N.M., Hardik Soni K., Bhatt S.B. (2012) Asian Journal of Biochemical and Pharmaceutical Research Issue,2 (2), 132-136.
19. Helen D., Graham R.B. (2002) Nature, 417,949–954.
20. Alireza S. (2016) J Pharma Care Health Sys, 3(2), 1-4.