COMPARATIVE STUDY BETWEEN BENZALKONIUM CHLORIDE TRI-SODIUM PHOSPHATE AND NALC-NAOH DECONTAMINATION METHODS FOR RECOVERY OF MYCOBACTERIUM TUBERCULOSIS FROM PULMONARY SAMPLE

S. BHIRANGE¹, R.N. MISRA², S. HATOLKAR³, N. GUPTA⁴, S. JADHAV⁵*
¹Department of Microbiology, Dr D.Y. Patil Medical College Hospital and Research Centre, Dr D. Y. Patil University, Pimpri, Pune, 411018, India
²Department of Microbiology, Dr D.Y. Patil Medical College Hospital and Research Centre, Dr D. Y. Patil University, Pimpri, Pune, 411018, India
³Department of Microbiology, Dr D.Y. Patil Medical College Hospital and Research Centre, Dr D. Y. Patil University, Pimpri, Pune, 411018, India
⁴Department of Microbiology, Dr D.Y. Patil Medical College Hospital and Research Centre, Dr D. Y. Patil University, Pimpri, Pune, 411018, India
⁵Department of Microbiology, Dr D.Y. Patil Medical College Hospital and Research Centre, Dr D. Y. Patil University, Pimpri, Pune, 411018, India
* Corresponding Author : patilsv78@gmail.com

Received : 01-12-2018     Accepted : 27-03-2019     Published : 30-03-2019
Volume : 11     Issue : 3       Pages : 1518 - 1520
Int J Microbiol Res 11.3 (2019):1518-1520

Keywords : Tuberculosis, Benzalkonium chloride method
Conflict of Interest : None declared
Acknowledgements/Funding : Authors are thankful to Department of Microbiology, Dr D.Y. Patil Medical College Hospital and Research Centre, Dr D. Y. Patil University, Pimpri, Pune, 411018, India
Author Contribution : All authors equally contributed

Introduction: Tuberculosis [TB] is a major public health concern worldwide over the last decades. About 80% of the global TB burden is in low income countries. Globally Multi drug resistant Tuberculosis caused an estimated 6 lack new TB cases and 2.40000 deaths in 2016.MDR TB accounts for 4.1% of all new TB and 19% of previously treated cases. Most of them occur in South America, Southern Africa, India, China and the former Soviet Union. Microbial diagnosis of TB consists of conventional and molecular methods but decontamination plays major role for perfect staining and cultures. Material and methods: 60 pulmonary samples were treated with benzalkonium chloride Tri-sodium phosphate and NALC-NaOH methods. All processed for ZN staining and LJ cultures were incubated at 37°C for 8 weeks. Results: Out of 60 respiratory clinical samples, 36 (60%) clinical samples were Z-N smear positive and 24(40%) were Z-N smear negative. Culture positivity was observed as 33 (55%) by NALC-NAOH method and 35 (58.3%) by Benzalkonium chloride method. Conclusion: Benalkonium chloride trisodium phosphate appears to be a reasonable incorporation in decontamination procedure and is fairly non-toxic to mycobacterium to improve growth in culture and a reasonably better mucolytic reagent.

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