NIGER SEED AGAR AS AN INDUCTOR OF SPORULATION OF FILAMENTOUS FUNGI WITH POTENTIAL IN BIOLOGICAL CONTROL OF AGRICULTURAL DISEASES AND PESTS

F.A.D. ITO¹, I. C. PIMENTEL², C.G. POITEVIN³*
¹Laboratory of Microbiology and Molecular Biology (LabMicro), Department of Basic Pathology, Sector of Biological Sciences, Federal University of Parana, 81530-900, Curitiba, Brazil
²Laboratory of Microbiology and Molecular Biology (LabMicro), Department of Basic Pathology, Sector of Biological Sciences, Federal University of Parana, 81530-900, Curitiba, Brazil
³Laboratory of Microbiology and Molecular Biology (LabMicro), Department of Basic Pathology, Sector of Biological Sciences, Federal University of Parana, 81530-900, Curitiba, Brazil
* Corresponding Author : arol.poitevin@gmail.com

Received : 23-02-2019     Accepted : 26-03-2019     Published : 30-03-2019
Volume : 11     Issue : 3       Pages : 1509 - 1513
Int J Microbiol Res 11.3 (2019):1509-1513

Keywords : Biocontrol, Culture medium, Photophase, Temperature
Academic Editor : Deepa P M, Dr Vinod Kumar Singh, Dr Poonam Choudhary, Raghu B.R., Manjot Kaur
Conflict of Interest : None declared
Acknowledgements/Funding : This study was financed by National Council for Scientific and Technological Development (CNPq) and National Council for the Improvement of Higher Education (CAPES). Authors are thankful to Federal University of Paraná, Curitiba, Brazil
Author Contribution : All authors equally contributed

The culture media Niger Seed Agar, Sabouraud Malt Yeast Extract Agar, Oat Agar, Rice Agar and Sabouraud were evaluated for the vegetative growth and sporulation of the entomopathogenic fungi Beauveria caledonica, Beauveria bassiana, Isaria javanica, Metarhizium anisopliae and the endophytic fungi Colletotrichum gloeosporioides, Botryosphaeria dothidea and Nectria pseudotrichia, all with potential against pests and diseases. The Niger Seed Agar presented a significant difference regarding sporulation in relation to the other media and its production cost was lower comparing to SMAY. Therefore, the Niger Seed Agar medium was selected for optimization of temperature and photophase. In general, the factorial analysis showed that these factors influenced significantly the growth of endophytic fungi and the sporulation of entomopathogens. This is the first study to demonstrate the efficiency of the Niger Seed Agar for sporulation of filamentous fungi. It is an economically viable alternative for the development of processes aiming the commercial production of bioinsecticides and biofungicides.

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