ISOLATION AND PHENOTYPIC CHARACTERIZATION OF NATIVE ENDOPHYTIC COFFEE BACTERIA (Coffea canephora P. var robusta) IN DALOA, COTE D IVOIRE

KOUASSI KOUASSI CLEMENT¹*, KONATE IBRAHIM², VOKO BI ROSIN DON RODRIGU³, ATSE MARIE-PAULE ABOUEUH^4
1Departement de Biochimie Microbiologie, Universite Jean Lorougnon Guede, Daloa, BP 150, Cote d Ivoire
²Departement de Biochimie Microbiologie, Universite Jean Lorougnon Guede, Daloa, BP 150, Cote d Ivoire
³Departement de Biochimie Microbiologie, Universite Jean Lorougnon Guede, Daloa, BP 150, Cote d Ivoire
⁴Departement de Biochimie Microbiologie, Universite Jean Lorougnon Guede, Daloa, BP 150, Cote d Ivoire
* Corresponding Author : clemankoici@yahoo.fr

Received : 21-02-2019     Accepted : 16-03-2019     Published : 30-03-2019
Volume : 11     Issue : 3       Pages : 1495 - 1502
Int J Microbiol Res 11.3 (2019):1495-1502

Keywords : Endophytic bacteria, Coffea canephora, Daloa
Conflict of Interest : None declared
Acknowledgements/Funding : Authors are thankful to the authorities of the village communities of the sampling sites as well as the brave producers for their frank collaboration. Authors are also thankful to Departement de Biochimie Microbiologie, Universite Jean Lorougnon Guede, Daloa, BP 150, Cote d Ivoire.
Author Contribution : All authors equally contributed

Endophytic bacteria could boost coffee production in Cote d Ivoire. This study evaluated the endophytic potentialities of coffee to be integrated into ecological engineering. Endophytes of Robusta have been isolated. Fifteen endophytes including 10 roots and 5 leaves were isolated. Bacillus, Actinomycetes and Pseudomonas have been identified. Ten isolates were tolerant to NaCl up to 10% and 3 isolates to KCl for the same concentrations. Thirteen isolates tolerated potassium nitrate up to 8% while sodium nitrate inhibited even 1%. These bacteria were able to grow in a pH range (4 to 12). With the exception of ciprofloxacin, other antibiotics have been tolerated up to at least 75 μgmL-1. Good tolerance to heavy metals up to 50 μgmL-1 was noted for at least 70% of strains. This study confirms the coffee tree as a natural host of endophytic bacteria, which would be well controlled to help the sustainable production of Robusta.

1. Sangaré A., Koffi E., Akamou F., Fall C.A. (2009) Etat des ressources phytogénétiques pour l’alimentation et l’agriculture de Côte d’Ivoire. FAO, second rapport national, 65
2. FAOSTAT (2015) http://faostat3.fao.org/browse/Q/_/F.
3. CCC (2017) Conseil Café-Cacao. 4ème édition des journées nationales du cacao et du chocolat, Abidjan, Côte d’Ivoire, 60.
4. Ducroquet H., Tillie P., Louhichi K., Gomez Y., Paloma S. (2017). EUR 28754 FR.
5. Esso L.J. (2009). PED N°10. CIRES. 19p.
6. Eponon C., Snoeck D., Kassin E., Keli J., Kone D. (2017) Cahiers Agricultures, 26, 45007.
7. Ruf F., Schroth G. (2013). Versailles: Éditions Quae,
8. Wairegi L.W., Van Asten P.J., Giller K.E., Fairhurst T. (2014) Banana-coffee system cropping guide. Africa Soil Health Consortium, Nairobi.
9. Dushimirimana S., Manirakiza E., Gasogo A. (2015) Bulletin scientifique sur l’environnement et la biodiversité, 1, 8-16.
10. Jha P.N., Gupta G., Jha P., Methrotra R. (2013) Greener Journal of Agricultural Sciences, 3, 73-84.
11. Cock M.J.W., Van Lenteren J.C., Brodeur J., Barratt B.I.P., Bigler F., Bolckmans K., (2009) FAO, Rome Italy: Commission on Genetic Resources for Food and Agriculture. Background Study Paper N°47.
12. Smaili MC., Ghadraoui L.E, Gaboun F., Benkirane R., Blenzar A. (2014) Phytoparasitica, 42(3), 421-436.
13. Crowder D.W. (2007) Journal of Insect Sciences, 7, 15.
14. Smaili M.C., Abbassi M., Boutaleb J.A., Blenzar A. (2013) EPPO Bulletin, 43(1), 155-166.
15. Kebe I.B, Mpika J., N’Guessan K.F., Hebbar P.K., Samuel G.S., Ake S. (2009) Sciences & Nature 6(1), 71-82.
16. Qin S., Xing K., Jiang J.H., Xu L.H., Li W.J. (2011) Applied Microbiology and Biotechnology, 89, 457-473.
17. Brader G., Compant S., Mitter B., Trognitz F., Sessitsch A. (2014) Current Opinion in Biotechnology, 27, 30-37.
18. Gomez L.C.C., Schiliro E., Valverde-Corredor A., Mercado-Blanco J. (2014) Frontiers in Microbiology, 5, 427.
19. Ji S.H., Gururani M.A., Chun S.C. (2014) Microbiological Research, 169, 83-98.
20. Santoyo G., Moreno-Hagelsieb G., Orozco-Mosqueda M.D.C., Glick B.R. (2016) Microbiological Research 183, 92-99.
21. Muzzamal H., Sarwar R., Sajid I. Hasnain S. (2012) Journal of Zoology, 44, 249-257.
22. Konaté I., Ouattara A., Coulibaly B., Guei R.N.K., Amani K., Kouadio K.I., Filali-Maltouf A., Koffi M. (2015) International Journal of Current Microbiology and Applied Sciences, 4(9), 560-570.
23. Priest F.G., Barker M., Baillie L.W., Holmes E.C., Maiden M. (2004) Journal of Bacteriology, 186(23), 7959-7970.
24. Shomura T., Yoshida J., Amano S., Kojima M., Inouye S., Niida T. (1979) Journal of Antibiotics (Tokyo). 32(5), 427-435.
25. Zhang Q., Achal V., Xiang W.N. Wang D. (2014) International Journal of Agriculture and Biology, 16: 619-623.
26. Miguel P.S.B., Delvaux J.C., Valério de Oliveira M.N., Monteiro L.C.P., Freitas F.S., Costa M.D., Tótola M.R., Alencar de Moraes C., Borges A.C. (2013) African Journal of Microbiology Research 7(7), 586-594.
27. Harllen S.A, Jaoa T., Wagner B. (2012) Biological Control, 63: [28] Teshome B., Wassie M., Abatneh E. (2017) Journal of Fertilizers & Pesticides, 8: 188. doi:10.4172/2471-2728.1000188.
29. Konaté I., Koulibaly A., Koffi M., Sokouri A., Berraho E.B., Filali-Maltouf A. (2014) American Journal of Life’s Sciences, 2, 325-332.
30. Chi F., Schi S.H., Cheng H.P., Jing Y.X., Yanni. Y.G. & Dazzo F.B. (2005) Applied and Environmental Microbiology, 71, 7271-7278.
31. Adejumo T.O., Orole O.O. (2010) Scientific Research and Essays, 5(13), 1655-1661.
32. Compant S., Clement C., Sessitsch A. (2010) Soil Biology Biochemistry, 42, 669-678.
33. Vega F.E., Pava-Ripoll M., Posada F., Buyer J.S. (2005) Journal of Basic Microbiology, 45(5): 371-380.
34. Reva O.N., Smirnov V.V., Pettersson B., Priest F.G. (2002) International Journal of Systematic and Evolutionary Microbiology, 52, 101-107.
35. Benyoub K. (2011) Mémoire de Magister, Biotechnologie végétale, Université des Sciences et de la technologie d’Oran « Mohamed BoudiaF », Algérie, 156 p.
36. Ghalem M. (2013) Mémoire de Magister, exploitation des interactions plantes-microorganismes, Université d’Orian Es-Sénia, Algérie, 137 p.
37. Desriani D., Kusumawati D.E., Rivai1 A., Hasanah N., Amrinola W., Triratna L., Sukma A. (2013) International Journal on Advanced Science Ehgineering Information Technology, 3, 76-78.
38. Amany M.R., Tharwat E.E.R., Khaulood A.H., Nabil A.A., Refaat M.A. (2017) International Journal of Current Science Resarch, 3(12): 1467-1488.
39. Sen D., Appanun C., Singh R.K. (2008) Indian Journal of Experimental Biology, 46, 846-851.
40. El Hilali I., J. El Jamali, I. Thami Alami, A. Filali Maltouf (2016) International Journal of New Technology and Research (IJNTR), 2(12): 27-37.
41. Khemili S. (2008) Mémoire de Magister en Biologie option Biochimie et microbiologie appliquées, Université M’hamed Bougara Boumerdes, Algerie, 168p.
42. Singh R.P., Jha P.N. (2015) International Journal of Current Microbiology Applied Sciences, 4, 142-152.
43. El Aafi N, Brhada F, Dary M, Filali Maltouf A., Pajuelo E. (2012). International Journal of Phytoremediation, 14(3), 261-274.
44. Patel P.R., Shaikh S.S., Sayyed R.Z. (2016) Indian Journal of Experimental. Biology, 54, 286-296.
45. Nonnoi F., Chinnaswamy A., García de la Torre V.S., Coba de la Peña T., Lucas M.M., Pueyo J.J. (2012) Applied Soil Ecology, 61, 49-59.
46. Le Mire G., Nguyen M.L., Fassotte B., Du Jardin P., Verheggen F., Delaplace P., Jijakli M.H. (2016) Biotechnology, Agronomy, Society and Environment, 20(S1), 299-313.