OPTIMIZATION OF THE TRANSIENT EXPRESSION OF CRSTR IN Ophiorrhiza mungos L. PLANTS BY AGRO INFILTRATION METHOD TO ENHANCE CAMPTOTHECIN PRODUCTION

NAGESHA B.V.¹*, UGRAIAH A.², RAVIKANTH G.³, K.N. NATARAJA⁴, UMASHAANKER R.^5
1Department of Crop Physiology, University of Agricultural Sciences, GKVK, Bengaluru, 560065, Karnataka, India
²School of Ecology and Conservation, University of Agricultural Sciences, GKVK, Bengaluru, 560065, Karnataka, India
³Ashoka Trust for Research in Ecology and the Environment, Royal Enclave, Srirampura, Jakkur PO, Bengaluru, 560064, Karnataka, India, India
⁴Department of Crop Physiology, University of Agricultural Sciences, GKVK, Bengaluru, 560065, Karnataka, India
⁵School of Ecology and Conservation, University of Agricultural Sciences, GKVK, Bengaluru, 560065, Karnataka, India
* Corresponding Author : nageshgowdabgh06@gmail.com

Received : 18-11-2018     Accepted : 27-12-2018     Published : 30-12-2018
Volume : 10     Issue : 12       Pages : 1425 - 1428
Int J Microbiol Res 10.12 (2018):1425-1428

Keywords : Camptothecin, Indole alkaloids, Agrobacterium tumefaciens, CrSTR gene
Conflict of Interest : None declared
Acknowledgements/Funding : Authors are thankful to University of Agricultural Sciences, GKVK, Bengaluru, 560 065, India
Author Contribution : All authors equally contributed

Camptothecin derivatives are clinically used as anti-tumor indole alkaloids that are currently obtained by extraction from intact plants. In the present study, we developed a method for transient expression of Catharanthus roseus strictosidine synthase (CrSTR) gene by Agro infiltration method in Ophiorrhiza mungos plant with Agrobacterium tumefaciens. Our results indicated that the construct in the LBA 4404 strain imparted a higher transient expression as evident by a higher accumulation of the end product, CPT (0.022 %) compared to the untransformed as well as the mock treatments. These results point at the possibility that overexpression of Strictosidine synthase could in principle lead to the overproduction of strictosidine which then can lead to the higher production of terpenoid indole alkaloids, such as camptothecin.

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