ISSN : 0975-5276
EISSN : 0975-9174
R.K. SHAH1*, H.B. GADEKAR2, SWETA KUMARI3, V. JAIN4, A. TRIPATHI5
1Department of Microbiology, RKDF Medical College Hospital and Research Centre, SRK University, Bhopal, 462026, Madhya Pradesh, India
2Department of Microbiology, RKDF Medical College Hospital and Research Centre, SRK University, Bhopal, 462026, Madhya Pradesh, India
3Department of Physiology, RKDF Medical College Hospital and Research Centre, SRK University, Bhopal, 462026, Madhya Pradesh, India
4Department of Microbiology, RKDF Medical College Hospital and Research Centre, SRK University, Bhopal, 462026, Madhya Pradesh, India
5Department of Microbiology, RKDF Medical College Hospital and Research Centre, SRK University, Bhopal, 462026, Madhya Pradesh, India
* Corresponding Author : rajesh_medmicro@yahoo.co.in
Received : 27-11-2018 Accepted : 27-12-2018 Published : 30-12-2018
Volume : 10 Issue : 12 Pages : 1422 - 1424
Int J Microbiol Res 10.12 (2018):1422-1424
Keywords : Potassium iodide, Sporothrix schenckii, Mycelial phase, Varying concentration, Colorimeter
Conflict of Interest : None declared
Acknowledgements/Funding : Authors are thankful to RKDF Medical College Hospital and Research Centre, SRK University, Bhopal, 462026, Madhya Pradesh, India
Author Contribution : All authors equally contributed
Introduction and Objective: Potassium iodide (KI) has been used as an effective drug for the treatment of sporotrichosis since centuries. Still it is one of the best drug fin the treatment of infections caused by Sporothrix schenckii, but its exact mechanism of action is unknown. Hence, it was aimed to study the effect of increasing concentration of KI on the normal growth curve of S. schenckii in mycelial phase. Material and Method: A MTCC 1359 strains of S. schenckii was used to study the effect of KI on its growth. A filamentous stage of this thermally dimorphic fungus was inoculated in broth culture medium i.e., yeast nitrogen base (YNB) medium and incubated at 25 ºC for 1 week. The broth culture was adjusted to turbidity of 90% transmittance at 530 nm in a colorimeter to obtain a master inoculum. A varying concentration of KI i.e., 0.05 gm%, 0.1g m% , 0.2 gm%, 0.4 gm%, 0.8 gm%, 1.6 gm%, 3.2 gm%, 6.4 gm%, 12.8 gm% and blank (0.00gm%) was added in ten tubes containing 5ml of YNB. All the tubes were inoculated with 0.1 ml of master inoculum and then incubated at 25ºC. Optical densities (ODs) of all the tubes were measured by taking readings of ODs at 530 nm of wavelength in a colorimeter for 34 days. Result: A significant change in ODs of all the phases of normal growth curve of mycelial form of S. schenckii was seen in increasing concentration of KI. The effect was more prominent in higher concentration of KI that too in exponential and decline phase of the normal growth curve than the lag and logarithmic phase. Conclusion: The study reveals an inhibitory effect of KI in its increasing concentration on normal growth curve of mycelial form of S. schenckii, but higher concentration had always shown the pronounced effect on all the phases of the normal growth curve. Hence, this study suggests that KI has an inhibitory activity on the growth of S. schenckii. percent.
1. Chander J. (2002) Textbook of Medical Mycology. Mehta publisher, second edition, 128-137.
2. Lopez-Romero E., Reyes-Montes M.R., Pérez-Torres A., Ruiz-Baca E., Villagómez-Castro J.C., Mora-Montes H.M., Flores-Carreón A. &Toriello C. (2011) Future Microbiology, 6,1, 85-102.
3. Kauffman C.A., Bustamante B., Champan S.W., Pappas P.G. (2007) IDSA guidelines for management of sporotrichosis, 45,1255-1265.
4. Orofino-Costa R., de Macedo P.M., Rodrigues A.M., Baernardes-Engemann A.R. (2017) Anais brasileiros dermatologia,92, 5, 606-620.
5. Barrows M.B.L., Paes R.A., Schubach A.O. (2011) Clinical Microbiol ogy Reviews, 24, 4, 633-654.
6. Rippon J. (1988) Medical mycology-he pathogenic fungi and the pathogenic actinomycetes, In J. Rippon (ed.), 3rd ed. W. B. Saunders Company, Philadelphia, PA, 325-352.
7. Shadomy S., Espinel-Ingroff A., Paxton L. (1978) Laboratory Methods in Medical Mycology, 4th edition, U.S. Department of health education and welfare, Public health services: Centre for disease control, Atlanta, Georgia, 173.
8. Shah R.K., Gadekar H.B., Jain V., Kumari S., Tripathi A., Gupta S. (2018) International Journal of Current Microbiology and Applied Sciences, 7,11, 1078-1084.
9. Bareja R., Grover P.S., Mehra S.K. (2015) Scholars Journals of Applied Medical Sciences, 2015,3,7D, 2676-2679.
10. Sterling J.B., Heymann W.R. (2000) Journal of the American Academy of Dermatology, 43, 4, 691-697.
11. Xue S.L., Li L. (2009) Mycopathologia, 167, 6, 355-356.