WOOD STIFFNESS AS A TRAIT FOR PHENOTYPING, SELECTIVE GENOTYPING AND PRELIMINARY GENETIC DIVERSITY ESTIMATION USING SSR MARKERS IN MELIA DUBIA

G. JOSHI¹, S.S. CHAUHAN², A.N. ARUNKUMAR³, T.L. AJAY⁴, C.N. SREEDEVI⁵, A. RAJAN⁶, D. ANNAPURNA⁷*
¹Tropical Forest research Institute, Jabalpur, Madhya Pradesh, 482021, India
²Institute of Wood Science and Technology, PO 18th Cross, Malleswaram, Bengaluru, Karnataka, 560 003, India
³Tropical Forest research Institute, Jabalpur, Madhya Pradesh, 482021, India
⁴Institute of Wood Science and Technology, PO 18th Cross, Malleswaram, Bengaluru, Karnataka, 560 003, India
⁵Institute of Wood Science and Technology, PO 18th Cross, Malleswaram, Bengaluru, Karnataka, 560 003, India
⁶Institute of Wood Science and Technology, PO 18th Cross, Malleswaram, Bengaluru, Karnataka, 560 003, India
⁷Institute of Wood Science and Technology, PO 18th Cross, Malleswaram, Bengaluru, Karnataka, 560 003, India
* Corresponding Author : uannapurna@gmail.com

Received : 30-10-2018     Accepted : 22-11-2018     Published : 30-11-2018
Volume : 10     Issue : 11       Pages : 541 - 547
Genetics 10.11 (2018):541-547

Keywords : Diversity, Melia dubia, polymorphism, selective genotyping, stress wave timer
Conflict of Interest : None declared
Acknowledgements/Funding : Financial support provided by University Grants Commission, New Delhi to Annapurna Dhavala, Karnataka Forest Department to Shakthi Singh Chauhan and Arun Kumar, Indian Council of Forestry Research and Education, Dehradun to Geeta Joshi is greatly acknowledged. We are grateful to the Director and the Group Coordinator of Research at the Institute of Wood Science and Technology, Bangalore for their encouragement and providing the necessary facilities. We sincerely acknowledge Karnataka State Forest Department and farmers for allowing us to visit the plantations of M. dubia in this study.
Author Contribution : Geeta Joshi was involved in supervision, planning methodology, field work, resources, partial funding acquisition, manuscript reviewing and editing. Chauhan Shakti Singh Arunkumar A. N. was involved in planning methodology for phenotyping, executing field

Trait specific phenotyping, selective genotyping and genetic diversity for wood stiffness was carried out in Melia dubia Cav. (Meliaceae), a native fast-growing tree species of India extensively used in plywood industry. Wood stiffness plays a major role for structural lumbers and veneer industry. To estimate it by non-destructive method, stress wave velocity was measured in standing trees (n=513) using a stress wave timer. The velocity ranged from 3.24 km/s to 4.24 km/s in trees with ≥ 37.5cm Girth at Breast Height (GBH). For selective genotyping, 20 genotypes having high (4.05- 4.46-km/s) and 27 genotypes having low (3.24- 3.94-km/s) stress wave velocity were selected. For genetic diversity estimates, 55 SSR markers were screened and 15 markers showing trait related polymorphism were selected. Analysis for genetic diversity revealed highly polymorphic loci (Na=22.2) and low level of observed heterozygosity (Ho= 0.18). In addition, the results showed positive fixation index of 0.81 which is an indicator of inbreeding. Twelve markers showed distinct dissimilarity between high and low stress wave velocity groups. Out of which, five markers, MSSR 26, 33, 37, 41 and Mc2 showed distinct alleles for high wood stiffness. Cluster analysis with 47 genotypes resulted in three major clusters of which 90% of genotypes were distinctly segregated into two clusters having either low or high wood stiffness character. Identifying more markers of above type may facilitate in future marker assisted selection programme for wood stiffness in M. dubia.

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