EVALUATION OF CULTURE MEDIA, LIGHT REGIMES AND NATURAL HOST SEGMENTS ON GROWTH AND SPORULATION OF Pyricularia oryzae Cavara CAUSING BLAST DISEASE IN RICE

B. BHASKAR¹, R.SARADA JAYALAKSHMI DEVI², S. VIJAY KUMAR³, C.P.D. RAJAN⁴, B. RAVINDRA REDDY⁵, M. SRINIVAS PRASAD⁶*
¹Department of Plant Pathology, ICAR-Indian Institute of Rice Research, Telangana, Hyderabad, 500030, India
²Department of Plant Pathology, S.V. Agricultural College, Tirupati, 517502, Acharya N. G. Ranga Agricultural University, Lam, 522034, Andhra Pradesh, India
³Department of Plant Pathology, ICAR-Indian Institute of Rice Research, Telangana, Hyderabad, 500030, India
⁴Department of Plant Pathology, Agricultural Research Station, Nellore, 524004, Acharya N. G. Ranga Agricultural University, Lam, 522034, Andhra Pradesh, India
⁵Department of Statistics and Computer Applications, S.V. Agricultural College, Tirupati, 517502, Acharya N. G. Ranga Agricultural University, Lam, 522034, India
⁶Department of Plant Pathology, ICAR-Indian Institute of Rice Research, Telangana, Hyderabad, 500030, India
* Corresponding Author : data.msprasad@gmail.com

Received : 10-10-2018     Accepted : 26-10-2018     Published : 30-10-2018
Volume : 10     Issue : 10       Pages : 1374 - 1377
Int J Microbiol Res 10.10 (2018):1374-1377

Keywords : Pyricularia oryzae Cavara, sporulation, different media, light regimes and host segments
Conflict of Interest : None declared
Acknowledgements/Funding : Authors are highly thankful to DST-INSPIRE fellowship programme, ICAR-Indian Institute of Rice Research, Telangana, Hyderabad, 500030, India. Author also thankful to Acharya N. G. Ranga Agricultural University, Lam, 522034, Andhra Pradesh, India
Author Contribution : All author equally contributed

The fungus Pyricularia oryzae Cavara (sexual phase Magnaporthe oryzae B.C.Couch) is the causal agent of rice blast disease. Sporulation in P. oryzae is a process distinct from mycelial growth in many respects and also difficult to get sporulation in P. oryzae. Finger millet leaf extract (FLA) and Rice leaf extract Agar (RLA) culture medium proved as best media with the sporulation of 1.71×105 ml-1 and 1.65×105 ml-1, respectively and no significant increase or decrease in sporulation was noticed under the three light regimes i.e., continuous fluorescent light, 12 hr dark + 12 hr light and continuous dark. Among all tested methods, the sporulation on Brachiaria mutica leaf and stem segments was found as best method with the sporulation of 7.61×105 ml-1.

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