CROSSABILITY STUDIES IN MANGO (Mangifera indica L.) MALFORMATION IN A SUBTROPICAL CLIMATE

M.K. VISHWAKARMA¹*, A. BAJPAI², C. Kole^3
1Department of Genetics and Plant Breeding, Sam Higginbottom University of Agriculture, Technology and Sciences, Allahabad, 211007, Uttar Pradesh, India
²Division of Crop Improvement and Biotechnology, ICAR-Central Institute for Sub-tropical Horticulture, Lucknow, Uttar Pradesh 226101, India
³Department of Genetics and Plant Breeding, Sam Higginbottom University of Agriculture, Technology and Sciences, Allahabad, 211007, Uttar Pradesh, India
* Corresponding Author : manishvis@gmail.com

Received : 28-09-2018     Accepted : 10-10-2018     Published : 30-10-2018
Volume : 10     Issue : 10       Pages : 511 - 513
Genetics 10.10 (2018):511-513

Keywords : Crossability, Pollen, Incompatibility, Floral, Malformation
Academic Editor : Amit Phonglosa
Conflict of Interest : None declared
Acknowledgements/Funding : Authors thankful to Division of Crop Improvement and Biotechnology, ICAR-Central Institute for Sub-tropical Horticulture, Lucknow, Uttar Pradesh 226101, India. Also thankful to Department of Genetics and Plant Breeding, Sam Higginbottom University of Agriculture, Technology and Sciences, Allahabad, 211007, Uttar Pradesh
Author Contribution : All author equally contributed

With a view to mine the reason behind mango malformation malady and its causes, a cytological study has been conducted. In this study, reproductive biology of normal and malformed flowers was studied in the subtropical conditions of India, using the cultivars ‘Dasehari’ and ‘Amrapali’. In this regard, characteristics of flower, pollen biology and cross compatibility of normal and malformed flowers were analyzed, using microscopy techniques from the beginning of the blooming season. Results showed a large variations in flower characteristics of malformed panicles. The flower size (diameter) was large and arrangement of sepals were varied. Carpel number and curved style shape were other major abnormalities. Whereas, pollen grains from malformed tissues were large rhomboid, inaperculate and had large pollen size with the varied germ pores in different frequencies. Acetocarmine and FDA staining also evidenced pollen sterility. In crossability study, pollen adhesion and germinability was poor of malformed pollen grains also the germinating pollen tubes were blocked by callose plugs. The malformed flowers when taken as female flowers were unable to affect pollen adhesion, probably due to their curved/hooked structure. These results showed that low fruit set in mango in subtropical climates is due to abnormal pollen and flowering pattern.

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