ASSESSMENT OF THE GENETIC DIVERSITY OF Dendrocalamus hamiltonii ALONG ALTITUDINAL GRADIENT

R.K. MEENA¹*, SHANTI², M.S. BHANDARI³, RAMA KANT⁴, S. BARTHWAL⁵, H.S. GINWAL^6
1Division of Genetics & Tree Improvement, Forest Research Institute, Dehradun, 248195, Uttarakhand, India
²Division of Genetics & Tree Improvement, Forest Research Institute, Dehradun, 248195, Uttarakhand, India
³Division of Genetics & Tree Improvement, Forest Research Institute, Dehradun, 248195, Uttarakhand, India
⁴Division of Genetics & Tree Improvement, Forest Research Institute, Dehradun, 248195, Uttarakhand, India
⁵Division of Genetics & Tree Improvement, Forest Research Institute, Dehradun, 248195, Uttarakhand, India
⁶Division of Genetics & Tree Improvement, Forest Research Institute, Dehradun, 248195, Uttarakhand, India
* Corresponding Author : rajnrcpb@gmail.com

Received : 10-09-2018     Accepted : 27-09-2018     Published : 30-09-2018
Volume : 10     Issue : 9       Pages : 501 - 506
Genetics 10.9 (2018):501-506

Keywords : Dendrocalamus hamiltonii, Bamboo, Genetic diversity, Microsatellite markers, Altitudinal gradient
Conflict of Interest : None declared
Acknowledgements/Funding : Authors are thankful to the Science Engineering and Research Board (SERB), New Delhi, India, for providing financial support. Director Forest Research Institute, Dehradun is also duly acknowledged for providing field and laboratory facilities. We are also thankful Rain Forest Research Institute Jorhat and Advanced Research Centre of Bamboo and Rattan, Aizawl for helping in sample collection.
Author Contribution : All author equally contributed

Dendrocalamus hamiltonii is a commercially important bamboo species of India, experiencing population depletion due to heavy extraction from natural forests. The present study has been taken up to generate the basic knowledge of the genetic diversity of the natural stands of D. hamiltonii along altitudinal gradient. Seventeen polymorphic markers developed in other bamboo species were validated in D. hamiltonii and used for genotyping the populations of different altitudinal class (low = 100m, medium = 600m and high = 1200m). A total of 109 alleles generated in 88 individuals of three populations revealed higher genetic diversity at species (h = 0.209, I = 0.339) and population level (h = 0.169, I = 0.269) which is comparable to other bamboo species. Despite of large proportion of the genetic variation confined within the populations (75%), significant level of genetic differentiation (PhiPT= 0.250 and GST =0.199) was observed among the populations. Relatively higher genetic diversity was exhibited by the populations located at higher altitude than the populations of middle and low altitude. Large number of private bands recorded in the higher altitude populations showed less gene flow among the populations across wide altitudinal range. Mantel test showed significant correlation between genetic distance and altitudinal distance (r2 = 0.315, p ≤ 0.010) and indicates that altitude also plays key role in influencing genetic differentiation of the populations of D. hamiltonii. The clustering pattern obtained in UPGMA dendrogram, PCoA plot and Structure analysis revealed that structuring among the populations is fairly good.

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