GENETIC DIVERSITY OF MELIA DUBIA USING ISSR MARKERS FOR NATURAL POPULATIONS AND PLANTATIONS

S. RAWAT¹, A.N. ARUNKUMAR², D. ANNAPURNA³, N.N. KARABA⁴, G. JOSHI⁵*
¹Genetics and Tree Improvement Division, Institute of Wood Science and Technology, 18th Cross Malleswaram, Bengaluru, 560003, Karnataka, India
²Genetics and Tree Improvement Division, Institute of Wood Science and Technology, Bengaluru, 560003, Karnataka, India; Genetics and Tree Improvement Division, Tropical Forest Research Institute, Jabalpur, 482021, Madhya Pradesh, India
³Genetics and Tree Improvement Division, Institute of Wood Science and Technology, 18th Cross Malleswaram, Bengaluru, 560003, Karnataka, India
⁴Department of Crop Physiology, University of Agricultural Sciences, GKVK, Bengaluru 560 065, Karnataka, India
⁵Genetics and Tree Improvement Division, Institute of Wood Science and Technology, Bengaluru, 560003, Karnataka, India; Genetics and Tree Improvement Division, Tropical Forest Research Institute, Jabalpur, 482021, Madhya Pradesh, India
* Corresponding Author : geejos@gmail.com

Received : 02-09-2018     Accepted : 17-09-2018     Published : 30-09-2018
Volume : 10     Issue : 9       Pages : 490 - 494
Genetics 10.9 (2018):490-494

Keywords : Melia dubia, ISSR markers, Genetic Diversity, Superior seed sources
Academic Editor : Rekha R Warrier
Conflict of Interest : None declared
Acknowledgements/Funding : Authors are thankful to Karnataka Forest Department and Indian Council of Forestry Research and Education for financial support. Authors are also thankful to the Director and the Group Coordinator of Research, Institute of Wood Science and Technology, 18th Cross Malleswaram, Bengaluru, 560003, Karnataka, India. We sincerely acknowledge Karnataka State Forest Department and farmers for allowing us to visit the forest areas and plantations for this study
Author Contribution : Swati Rawat was involved in sample collection, laboratory work for genetic diversity, data curation, formal analysis, writing original draft. Arunkumar A. N. carried out field work, partial funding acquisition and manuscript reviewing and editing. Annap

Melia dubia Cav. (Family: Meliaceae) is a deciduous tree species native to India. It is an important plantation species with short rotation and multipurpose uses, highly valued as a pulpwood and plywood. Genetic variation was assessed in eleven natural populations and seven plantations across eight districts of Karnataka comprising 232 samples through 15 ISSR markers. At species level, genetic diversity estimates viz., Percentage polymorphism (94.6), percentage of polymorphic loci (PPL) (98.8), observed number of alleles (Na=1.98), effective number of alleles (Ne= 1.59), Neis gene diversity (H) (0.34±0.15) and Shannons information index (I) (0.51±0.19) were found to be high. In individual populations H ranged from 0.10±0.19 to 0.32±0.18 and I ranged from 0.15±0.26 to 0.47±0.25. Among different natural populations, Bhadravati exhibited the highest level of variability while in plantations Hunsur had maximum variability. Analysis of Molecular Variance showed that much of the genetic variation resided within the populations (68%) than among populations. The dendrogram obtained by using Unweighted Pair- Group method with Arithmetic average did not reflect geographical sub clustering of genetic diversity except for few populations. Based on the genetic variability found, superior seed sources can be identified and tree improvement strategies could be developed for conservation and further improvement of the species.

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