ISSN : 0975-2862
EISSN : 0975-9158
FATIMA TANZEEM1*, ASHUTOSH SRIVASTAVA2, P.V. SOMASHEKAR3, VAGEESHBABU S. HANUR4
1Tree Improvement and Genetics Division, Institute of Wood Science and Technology, Bangalore, 560003, India
2Tree Improvement and Genetics Division, Institute of Wood Science and Technology, Bangalore, 560003, India
3Tree Improvement and Genetics Division, Institute of Wood Science and Technology, Bangalore, 560003, India
4Division of Biotechnology, ICAR-Indian Institute of Horticultural Research, Hessaraghatta Lake Post, Bangalore, 560089, India
* Corresponding Author : tanzeem.fatima@gmail.com
Received : 09-06-2018 Accepted : 27-06-2018 Published : 30-06-2018
Volume : 10 Issue : 6 Pages : 443 - 451
Genetics 10.6 (2018):443-451
Keywords : Genomic DNA, timbers, phenolic, ISSR, SSR
Conflict of Interest : None declared
Acknowledgements/Funding : Authors are thankful to the Director, Group Coordinator Research, Head- Tree Improvement and Genetics Division, Institute of Wood Science and Technology and The Additional Principal Chief Conservator of Forest (research) the Karnataka Forest Department for financial support
Author Contribution : All author equally contributed
Dalbergia latifolia and Lagerstroemia lanceolata are two economically important and expensive timber species of tropical countries (India, Indonesia and Madagascar, Burma) are exploited. An efficient and on site detection and legal confirmation of these timbers is possible using molecular methods, but molecular tools have not been employed due to lack of knowledge of DNA extraction and species specific identification. Purified genomic DNA, required for many applications in molecular genetics is frequently more difficult to obtain from trees than most other crop or annual plants. Here we describe the essential steps of a rapid DNA isolation protocol that can be used for diverse timber species, which contains huge amount of secondary metabolites such as alkaloids, flavonoids, phenols, gummy polysaccharides, terpenes and quinones. This modified DNA extraction protocol is applicable for fresh and mature dried leaf samples. This customized procedure has improved the efficiency of genomic DNA by enhancing the yield as well as purity of DNA that would facilitate efficient characterization and validation of these timber species. The isolated DNA has been proved to be acquiescent to PCR amplification though ISSR and SSR primers.
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