ISSN : 0975-5276
EISSN : 0975-9174
V.L. KULKARNI1*, A.G. KINIKAR2, D.S. BHALERAO3, S.C. DEORUKHKAR4, S.B. ROUSHANI5
1Department of Microbiology, Rural Medical College, Pravara Institute of medical Sciences, (Deemed University) Loni, Rahata, Maharashtra 413736, India
2Department of Microbiology, Rural Medical College, Pravara Institute of medical Sciences, (Deemed University) Loni, Rahata, Maharashtra 413736, India
3Department of Microbiology, Rural Medical College, Pravara Institute of medical Sciences, (Deemed University) Loni, Rahata, Maharashtra 413736, India
4Department of Microbiology, Rural Medical College, Pravara Institute of medical Sciences, (Deemed University) Loni, Rahata, Maharashtra 413736, India
5Department of Microbiology, Rural Medical College, Pravara Institute of medical Sciences, (Deemed University) Loni, Rahata, Maharashtra 413736, India
* Corresponding Author : kvlvishal@gmail.com
Received : 29-05-2018 Accepted : 22-06-2018 Published : 30-06-2018
Volume : 10 Issue : 6 Pages : 1279 - 1282
Int J Microbiol Res 10.6 (2018):1279-1282
DOI : http://dx.doi.org/10.9735/0975-5276.10.6.1279-1282
Keywords : Clinical Microbiology, Bacteriology, Acinetobacter baumannii, Nonfermenters, Multi-drug resistance, ICU infections
Academic Editor : Dr Santosh Tathe, Dr Nagesh Abdagire
Conflict of Interest : None declared
Acknowledgements/Funding : Author thankful to Rural Medical College, Pravara Institute of medical Sciences, (Deemed University) Loni, Rahata, Maharashtra 413736, India
Author Contribution : All author equally contributed
Introduction-Infections due to Acinetobacter baumannii has become a global medical challenge. Multidrug resistant Acinetobacter baumannii isolates are causing catastrophic consequences in healthcare settings particularly in the critical care areas. The purpose of this study was to isolate A. baumannii species from different clinical specimens and to determine their antimicrobial resistance pattern. Methodology- The study was done in Department of Microbiology, RMC, Loni. The clinical samples were subjected to Gram staining, aerobic culture using MacConkey and blood agar. Acinetobacter baumannii species were identified by standard microbiological identification methods. Antimicrobial susceptibility testing was done by Kirby Bauer disc diffusion technique. Result- A total of 114 Acinetobacter baumannii species were isolated. Maximum number of Acinetobacter baumannii isolates were recovered from pus sample (41.23%) followed by tracheal secretions (30.7%). Rate of isolation of A. baumannii was highest from critical care areas (42.11%). Highest resistance was noted against Tobramycin (100%) followed by Cefotaxim (98.4%), Piperacillin (96.8%), and Ceftazidime (96.2%). All strains were sensitive to Polymyxin B & Colistin. Multidrug resistant A. baumannii isolates were found to be 77.19%. Conclusion- Stringent infection control protocols, regular practice of hand hygiene and strict implementation of antibiotics policy may help to control multidrug resistant ICU bugs.
1. Howard A., O’Donoghue M., Feeney A., Sleator D. (2012) Virulence,3(3),243–50.
2. Bergogne-Berezin E., Towner K.J. (1996) Clin Microbiol Rev,9,148–65.
3. Abbo A., Navon-Venezia S., Hammer-Muntz O., Krichali T., Siegman-Igra Y., Carmeli Y.(2005) Emerg Infect Dis,11(1),22–9.
4. Kulkarni V., Kinikar A., Bhalerao D., Deorukhkar S., Roushani S.(2017) IntJCurrMicrobiolAppSci,6(9),1103–11.
5. Perez F., Hujer A., Hujer K., Decker B., Rather P., Bonomo R.(2007) Antimicrob Agents Chemother,51(10),3471–84.
6. Kinikar A., Bhalerao D., Roushani S. (2013) Indian Med Gazzette,(11),420–3.
7. Fournier P.E., Richet H. (2006) Clin Infect Dis,42,692–9.
8. Muthuswamy D., Boppe A. (2012) J Clin Diagnostic Res,6(6),970–3.
9. Altun H., Yagci S., Bulut C., Sahin H., Kinikli S., Adiloglu A., Demiroz A. (2014) J Microbiol,7(12),01–4.
10. Washington C.W., Stephen D.A., William M.J., Koneman E.W., Procop G.W., Schreckenberger P.C. (2006) 6th ed. Philadelphia, Lippincott Williams & Wilkins.
11. Clinical and Laboratory Standards Institute (CLSI) Performance Standards for Antimicrobial Susceptibility Testing. 26th ed. CLSI supplement M100S, ISBN 1-56238-923-8.
12. Maragakis L., Perl T. (2008) Antimicrob Resist CID,46,1254–63.
13. Talukdar A., Hodiwala A., Sharma R. (2018) Int.J Curr Microbiol App Sci, 7(2),1176–86.
14. Peleg A.Y., Seifert H. & Paterson D.L. (2008) Clin Microbiol Rev, 21,538– 582.
15. Wisplinghoff H., Perbix W. & Seifert H. (1999) Clin Infect Dis., 28,59–66.
16. Antunes L.C.S., Visca P., Towner L.K. (2014) Pathogens & Disease,71,292–301.
17. Ghaima K.K., Saadedin S.M.K., Jassim K.A. (2016) Int J Sci Res Publ,6(5),351–6.
18. Jafari R., Karbasizade V. (2014) Advances in Bioresearch, 5 (2), 148-152.
19. Norouzi J., Heydarpour A., Mohammadi M. (2003) Archive of SID, 9(24), 1-5.
20. Marais E., de Jong G., Ferraz V., Maloba B. and Duse A. G. (2004) South Africa Am J Infect Control,32,278–281.
21. Jaggi N., Sissodia P., Sharma L. (2012) J Microbiol Infect Dis,2(2),57–63.
22. Chang W.N., Lu C.H., Huang C.R. & Chuang Y.C. (2000) Infection,28,395–397.
23. Falagas M.E., Karveli E.A., Kelesidis I. & Kelesidis T.(2007) Eur J Clin Microbiol Infect Dis, 26,857–868.
24. Gaur A., Garg A., Prakash P., Anupurba S., and Mohapatra T.M. (2008) J Health Popul N, 26 (2), 183-188
25. Dent L., Marshall D, Pratap S, Huelette R. (2010) BMC Infect Dis,10(196),01–7.
26. Castilho S.R.A., Godoy C.S.dM., Guilarde A.O., Cardoso J.L., Andre M.C.P., Junqueira-Kipnis A.P., et al. (2017) PLoS ONE 12(5).