ISSN : 0975-5276
EISSN : 0975-9174
POONAM BHAGAT1*, S.K. GOSAL2
1Department of Microbiology, Punjab Agricultural University, Ludhiana, Punjab, 141003, India
2Department of Microbiology, Punjab Agricultural University, Ludhiana, Punjab, 141003, India
* Corresponding Author : poonambhagat11.pb@gmail.com
Received : 20-04-2018 Accepted : 25-04-2018 Published : 30-04-2018
Volume : 10 Issue : 4 Pages : 1155 - 1159
Int J Microbiol Res 10.4 (2018):1155-1159
DOI : http://dx.doi.org/10.9735/0975-5276.10.4.1155-1159
Keywords : Biochemical characterization, cellulolytic bacteria, cellulolytic activity, morphological characterization and rice straw
Conflict of Interest : None declared
Acknowledgements/Funding : Author thankful to Punjab Agricultural University, Ludhiana, Punjab, 141003, India
Author Contribution : All author equally contributed
In this study, seventeen bacterial cultures were isolated and screened for cellulose degradation activity. The maximum halozone was observed with SKPB3 (2.8 mm). These isolates were then studied morphologically and most of them showed sticky consistency. Biochemical characterization showed most of them are oxidase and catalase positive and negative for H2S test, indole test and MR-VP test and few were positive for citrate utilization, gelatin solubilization and starch hydrolysis. The functional characterization of isolates showed highest cellulolytic activity (1.44 μg/ml), IAA production (34.85μg/ml), ammonia excretion (2.048μg/ml) and phosphate solubility (28.56 μg/ml) was exhibited by isolate SKPB3. The results revealed that the wheat field incorporated with rice straw contained bacteria which had ability to degrade the cellulose or they have ability to utilize the cellulose.
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