INCIDENCE AND ANTIMICROBIAL SUSCEPTIBILITY OF ENTEROCOCCAL INFECTIONS IN TERTIARY CARE HOSPITAL

KALPANA ANGADI¹, SAVITA JADHAV²*, R.N. MISRA³, SHAHZAD BEG MIRZA⁴, DEEPALI DESAI^5
1Department of Microbiology, Dr D. Y. Patil Medical College Hospital and Research Centre, Dr D.Y. Patil Vidyapeeth, Pimpri, Pune, 411018, India
²Department of Microbiology, Dr D. Y. Patil Medical College Hospital and Research Centre, Dr D.Y. Patil Vidyapeeth, Pimpri, Pune, 411018, India
³Department of Microbiology, Dr D. Y. Patil Medical College Hospital and Research Centre, Dr D.Y. Patil Vidyapeeth, Pimpri, Pune, 411018, India
⁴Department of Microbiology, Dr D. Y. Patil Medical College Hospital and Research Centre, Dr D.Y. Patil Vidyapeeth, Pimpri, Pune, 411018, India
⁵Department of Microbiology, Dr D. Y. Patil Medical College Hospital and Research Centre, Dr D.Y. Patil Vidyapeeth, Pimpri, Pune, 411018, India
* Corresponding Author : patilsv78@gmail.com

Received : 12-03-2018     Accepted : 12-04-2018     Published : 30-04-2018
Volume : 10     Issue : 4       Pages : 1135 - 1138
Int J Microbiol Res 10.4 (2018):1135-1138
DOI : http://dx.doi.org/10.9735/0975-5276.10.4.1135-1138

Keywords : VRE, Enterococcus faecalis, Enterococcus faecium, HA-UTI
Conflict of Interest : None declared
Acknowledgements/Funding : Author thankful to Department of Microbiology, Dr D. Y. Patil Medical College Hospital and Research Centre, Dr D.Y. Patil Vidyapeeth, Pimpri, Pune, 411018, India
Author Contribution : All authors equally contributed

Introduction: Enterococci have become second most common cause of hospital acquired Urinary tract infections [HA-UTIs] and wound infections and third leading cause of bacteremia. In recent years; Enterococci recognized with increasing frequency as common cause of Intra abdominal and pelvic infections, post surgery wound infections, and endocarditis. Most infections are caused by two enterococcal species i.e., Enterococcus faecalis and Enterococcus faecium. Early detection of resistant strains will help in the institution of appropriate therapy and also helps to lessen treatment failure, selection and spread of resistant strains. Materials and methods: The study was a Cross sectional study and was conducted in the Department of Microbiology at a tertiary care health centre in Western Maharashtra over a period of one year from July 2015 to June 2016. Enterococci were isolated on Blood agar plate as non-haemolytic 0.5–1mm size streptococci-like colonies; and on CLED agar as small yellow colonies from fermentation of lactose. Identification was done by standard conventional methods. The susceptibility was determined using Clinical and Laboratory Standards Institute (CLSI) disk diffusion method (CLSI, 2007) Identification and isolation with VITEK 2C AUTOMATED SYSTEM were done for Enterococci, Daptomycin Epsilometer test (E test) were done to determine MIC. Results and Observations: A total 141 [100%] Enterococci species were isolated from various clinical infections; of which 58.15% strains were from UTIs, 324.11% were from wound and pyogenic infections, 10.63% were from various body fluids while 3.54% were from bacteremia and respiratory infections. Of which 141; 54.6% enterococcal strains were isolated from female patients and 45.4% were from male patients. The age group 20–60 years constitute the largest proportion 85 (60%) followed by age group 1- 12 years 30 (21.3%) and in Elderly [≥ 60yrs] 26 [18.4%]. Of the total 141 clinical isolates of Enterococci; 15.60 % isolates were from outdoor patients, 84.39% admitted in hospital; 22% from various ICUs. E. faecalis were 48 %, E. faecium in 47 %, E. gallinarum 3 %, E. avium in 1 %. In our study, VRE was seen in approximately 2 %. All E. faecalis were sensitive to Vancomycin, Teicoplanin, Linezolid, Tigecycline and Daptomycin. Conclusion: High level resistance was detected in aminoglycoside, penicillin, quinolones in the present study though the prevalence rate of VRE is low. In recent years; changing pattern of Enterococcus spp. as a causative agent in clinical infections should be consider as E. faecium with high level resistance is more prevalent in developing countries.

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