EVALUATION OF MONOCROTOPHOS DEGRADATION BY Lysinibacillus sphaericus JX012235 ISOLATED FROM SWINE WASTE

S. MEIGNANALAKSHMI¹*, J. DEEPIKA², M. CHARULATHA³, K. VIJAYARANI^4
1Department of Animal Biotechnology, Madras Veterinary College, Tamil Nadu Veterinary and Animal Sciences University, Chennai, 600007, Tamil Nadu
²Department of Biotechnology, SRM University, Kattankulathur, Chennai, Tamil Nadu 603203
³Department of Animal Biotechnology, Madras Veterinary College, Tamil Nadu Veterinary and Animal Sciences University, Chennai, 600007, Tamil Nadu
⁴Department of Animal Biotechnology, Madras Veterinary College, Tamil Nadu Veterinary and Animal Sciences University, Chennai, 600007, Tamil Nadu
* Corresponding Author : smeignanalakshmi@gmail.com

Received : 25-01-2018     Accepted : 17-02-2018     Published : 28-02-2018
Volume : 10     Issue : 2       Pages : 1011 - 1014
Int J Microbiol Res 10.2 (2018):1011-1014
DOI : http://dx.doi.org/10.9735/0975-5276.10.2.1011-1014

Keywords : Monocrotophos, Lysinibacillus sphaericus, Biodegradation, Swine waste, Phosphatase activity
Academic Editor : B.L. Raghunandan, Trupti B. Naik
Conflict of Interest : None declared
Acknowledgements/Funding : Author are thankful to Madras Veterinary College, Tamil Nadu Veterinary and Animal Sciences University, Chennai, 600007, Tamil Nadu
Author Contribution : All author equally contributed

In India, the mainly used pesticide in agricultural crops is Monocrotophos, an organophosphorus pesticide. It is denoted as extremely toxic and their exposure to environment is considered to be lethal. In this study, degradation of Monocrotophos by Lysinibacillus sphaericus JX012235 a bacterium isolated from swine waste was studied. Growth studies of the bacteria in mineral salt broth supplemented with the pesticide at the concentration of 100-1000 ppm initially confirmed the degradation of the pesticide. The bacterium was found to survive when pesticide was given as a sole source of nutrition. The pesticide degradation was confirmed by LC-MS. The phosphatase activity of bacterium was found to be 69U/ml.

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