PREVALENCE AND GENOTYPE DIVERSITY OF ANAL HPV INFECTION AMONG HIV-INFECTED WOMEN IN BELEM, PARA, NORTH OF BRAZIL

L.L.S. RODRIGUES¹, A.R.B. SILVA², J.C. MONTEIRO³, R.V. LAURENTINO⁴, R.V.D. SILVESTRE⁵, V.N. AZEVEDO⁶, R.N.M. FEITOSA⁷, A.C.R. VALLINOTO⁸, R. ISHAK⁹, L.F.A. MACHADO¹⁰*
¹Laboratorio de Virologia, Instituto de Ciencias Biologicas, Universidade Federal do Para, Belem, Para, Brazil
²Laboratorio de Virologia, Instituto de Ciencias Biologicas, Universidade Federal do Para, Belem, Para, Brazil
³Laboratorio de Virologia, Instituto de Ciencias Biologicas, Universidade Federal do Para, Belem, Para, Brazil
⁴Laboratorio de Virologia, Instituto de Ciencias Biologicas, Universidade Federal do Para, Belem, Para, Brazil
⁵Laboratorio de Virologia, Servico de Vigilancia em Saude, Ministerio da Saude, Instituto Evandro Chagas, Belem, Para, Brazil
⁶Laboratorio de Virologia, Instituto de Ciencias Biologicas, Universidade Federal do Para, Belem, Para, Brazil
⁷Laboratorio de Virologia, Instituto de Ciencias Biologicas, Universidade Federal do Para, Belem, Para, Brazil
⁸Laboratorio de Virologia, Instituto de Ciencias Biologicas, Universidade Federal do Para, Belem, Para, Brazil
⁹Laboratorio de Virologia, Instituto de Ciencias Biologicas, Universidade Federal do Para, Belem, Para, Brazil
¹⁰Laboratorio de Virologia, Instituto de Ciencias Biologicas, Universidade Federal do Para, Belem, Para, Brazil
* Corresponding Author : lfam@ufpa.br

Received : 16-11-2017     Accepted : 06-01-2018     Published : 30-01-2018
Volume : 10     Issue : 1       Pages : 993 - 996
Int J Microbiol Res 10.1 (2018):993-996
DOI : http://dx.doi.org/10.9735/0975-5276.10.1.993-996

Keywords : HPV, Genotypes¸ Diversity, Anal, HIV
Academic Editor : Dedwal Ashwini Kacharu, Dr Vinod Kumar Singh
Conflict of Interest : None declared
Acknowledgements/Funding : We acknowledge all subjects enrolled in the present study and PROPESP/UFPA
Author Contribution : All the authors equally contributed

The objective of this cross-sectional study was to determine the prevalence of anal HPV infection, genotype diversity and its relationship with demographic aspects of HIV-infected assisted in Belém, Pará, Brazil. A questionnaire regarding socio-demographic variables and sexual behavior was used and anal samples were collected from 108 women recruited from a HIV Counselling and Testing in the Belém, Pará, an important metropolis of the northern region of Brazil. A blood sample was also obtained to determine CD4+ T lymphocytes counts and viral load levels of HIV. HPV DNA was detected by PCR amplification of a conserved region of the HPV L1 gene and further genotyped with direct DNA sequencing. HPV infection was detected in 29 (26,8%). Twelve different types were found (HPV-16, 58, 59, 6, 11, 53, 61, 62, 66, 70, 71, 102) and low risk types were present in 72.4% of samples. HPV infection was positively correlated with CD4+ T lymphocytes counts. In conclusion, we found a low prevalence of HPV infection and low risk types in this cohort and we emphasize the relevance of implementation of screening program for anal cancer and HPV infection in the HIV-infected women population.

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