GENEXPERT FOR GASTRIC LAVAGE TO DIAGNOSE PULMONARY TUBERCULOSIS IN CHILDREN

J.A. IRAVANE¹, AMBREEN FATEMA SHAIKH²*, AJIT S. DAMLE^3
1Department of Microbiology, Government Medical College, Aurangabad, 431004, Maharashtra University of Health Sciences, Nashik, Maharashtra
²Department of Microbiology, Government Medical College, Aurangabad, 431004, Maharashtra University of Health Sciences, Nashik, Maharashtra
³Department of Microbiology, Government Medical College, Aurangabad, 431004, Maharashtra University of Health Sciences, Nashik, Maharashtra
* Corresponding Author : dr.ambreen1212@gmail.com

Received : 25-10-2017     Accepted : 07-11-2017     Published : 28-11-2017
Volume : 9     Issue : 11       Pages : 1 - 4
Int J Microbiol Res 9.11 (2017):1-4

Keywords : Mycobacterium Tuberculosis, Gastric Lavage, Ziehl-Neelsen staining, GeneXpert
Conflict of Interest : None declared
Acknowledgements/Funding : Author are thankful to Department of Microbiology, Government Medical College, Aurangabad, 431004, Maharashtra University of Health Sciences, Nashik, Maharashtra
Author Contribution : All author equally contributed

Rapid and accurate diagnosis of pulmonary tuberculosis in children is still a unique challenge because of difficulties in obtaining sputum samples and the paucibacillary nature of the disease. Objective of this study was to evaluate the utility of GeneXpert on Gastric lavage samples in children. This prospective study was conducted over a period of one and half year i.e. from January 2016 to June 2017 in TB C & DST Laboratory, Department of Microbiology, Government Medical College, Aurangabad. During this period, 486 Gastric lavage samples of suspected Pediatric TB were referred to our laboratory. We processed by three methods which are microscopy, GeneXpert and Culture. Of the 486 GL samples, 5 (1.02%) were smear positive; 23 (4.73%) were GeneXpert positive and 7 (1.44%) were culture positive. In GeneXpert, one sample (4.34%) showed resistance to Rifampicin. In our study sensitivity of conventional methods in GL samples of children with pulmonary MTB was low as compared to GeneXpert. GeneXpert with advantages of quick turnaround time, simultaneously detecting rifampicin resistant TB provides a promising solution to the TB diagnostic challenges in children.

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