GLYCEROL BIOCONVERSION INTO 1,3-PROPANEDIOL AND 2,3-BUTANEDIOL BY Lactuca sativa RHIZOBACTERIA

RENAN DE SOUZA SOARES¹*, MARCUS VINICIUS FORZANI², ARIANA ALVES RODRIGUES³, BRUNO FRANCESCO RODRIGUES DE OLIVEIR⁴, KELLY JOHANA DUSSAN MEDINA⁵, SILVIO SILVERIO DA SILVA⁶, JOSE DANIEL GONCALVES VIEIRA^7
1Laboratory of Environmental Microbiology and Biotechnology (LAMAB), Federal University of Goias, Goiania, Goias, 74605050, Brazil
²Laboratory of Environmental Microbiology and Biotechnology (LAMAB), Federal University of Goias, Goiania, Goias, 74605050, Brazil
³Laboratory of Environmental Microbiology and Biotechnology (LAMAB), Federal University of Goias, Goiania, Goias, 74605050, Brazil
⁴Laboratory of Environmental Microbiology and Biotechnology (LAMAB), Federal University of Goias, Goiania, Goias, 74605050, Brazil
⁵Biotechnology Department, Engineering School of Lorena, University of Sao Paulo, Lorena, Sao Paulo, 12602810, Brazil
⁶Biotechnology Department, Engineering School of Lorena, University of Sao Paulo, Lorena, Sao Paulo, 12602810, Brazil
⁷Laboratory of Environmental Microbiology and Biotechnology (LAMAB), Federal University of Goias, Goiania, Goias, 74605050, Brazil
* Corresponding Author : renan_souza37@hotmail.com

Received : 07-09-2017     Accepted : 20-09-2017     Published : 28-09-2017
Volume : 9     Issue : 9       Pages : 954 - 958
Int J Microbiol Res 9.9 (2017):954-958

Keywords : Bioenergy, Fermentation, Byproduct, Carbon source, Industrial microbiology
Academic Editor : Lilian Carla Carneiro
Conflict of Interest : None declared
Acknowledgements/Funding : The authors thank the Research Support Foundation of the State of Goias (Fundacao de Amparo a Pesquisa do Estado de Goias - FAPEG), process number 201210267001173 and the Coordination for the Improvement of Higher Education Personnel (CAPES) for the fellowship to the first author.
Author Contribution : all author equally contributed

The present work aimed to isolate Lactuca sativa (lettuce) rhizobacteria and assess its capacity to bio-convert glycerol into the added-value compounds 2,3-butanediol and 1,3-propanediol. Six strains were isolated from rhizospheric soil in a selected culture medium and identified by sequencing the 16S rDNA region. The microbial growth pattern was tested in different glycerol concentrations, and the simultaneous production of the compounds of interest was quantified. The species of the Enterobacteriaceae family dominated, especially the genus Enterobacter. The test of different glycerol concentrations in culture medium suggests a concentration of 20 g.L-1 as ideal to promote the fermentation process and grow the isolated rhizobacteria. Three samples were identified as simultaneous producers of the relevant compounds. The best fermenting isolate, Enterobacter cloacae (AG3), produced 0.522 g.L-1 of 2,3-butanediol and 0.735 g.L-1 of 1,3-propanediol. Therefore, lettuce rhizobacteria are capable of producing added-value compounds from the fermentation of glycerol as a sole carbon source.

[1] C., Luque R., Luna D., Hidalgo J.M., Posadillo A., Sancho E.D., Rodriguez S., Ferreira-Dias S., Bautista F. and Romero A.A. (2010) Bioresour Technol, 101(17), 6657-6662. [2] (2013) Anuário estatístico brasileiro do petróleo, gás natural e biocombustíveis: 2013. ANP, Rio de Janeiro. [3] O., Horvath T., Pond C., Misra M. and Mohanty A. (2015) Ind Crops Prod, 78, [4] W.S., Kang J.H., Chu H.S., Choi I.S. and Cho K.M. (2014) Metab Eng, 23, 116-122. [5] J.R., Fávaro L.C. and Quirino B.F. (2012) Biotechnol Biofuels, 5(1), [6] K. and Trchounian A. (2015) Appl Energ, 156, [7] M., Paramithiotis S., Drosinos E.H., Galiotou‐Panayotou M., Nychas G.J.E., Zeng A.P. and Papanikolaou S. (2012) Eng Life Sci., 12(1), [8] R., Hartmann A. and Nehls U. (2012) In: Matyssek R, Schnyder H, Oßwald W, Ernst D, Munch JC, Pretzsch H (eds) Growth and defence in plants. Berlim, Springer Berlin [9] B.H., Lee B., Kloepper J.W. and Ryu C.M. (2013) Plant Signal Behav., 8(7), e24619. [10] C.M. (2015) Principles of Plant-Microbe Interactions. Switzerland, Springer International Publishing. [11] M.H., Kim S.J., Kim J.W., Park Y.C. and Seo J.H. (2016) Process Biochem, 51(2), [12] D. and Leja K. (2014) Electron J Biotechnol, 17(2), [13] B., Yonsel S. and Deckwer W.D. (1991) Appl Microbiol Biotechnol, 36(3), [14] A.A., Forzani M.V., Soares R.D.S., Sibov S.T. and Vieira J.D.G. (2016) Pesqui Agropecu Trop, 46(2), 149-158. [15] O.V.D., Rosa D.D. and Camargo L.E.A. (2008) Sci Agric., 65(5), [16] L.D. and Haas H.F. (1941) ‎J. Bacteriol, 41(5), [17] M.M (1936) J Pathol, 42 (2), 441-454. [18] S.M., Khan A.L., Hussain J., Ali L., Kamran M., Waqas M. and Lee I.J. (2012) Molecules 17(7), [19] J, Hernández JA, Caravaca F and Roldán A (2009) Environ Exp Bot, 65(2), 245-252. [20] C.C., Kämpfer P., Shen F.T., Lai W.A. and Arun A.B. (2005) Int J Syst Evol Microbiol, 55(1), [21] C.K., Aeron A., Patel B.V., Maheshwari D.K. and Saraf M. (2011) In: Maheshwari, D. K. (ed) Bacteria in Agrobiology: Plant Growth Responses. Berlin, Springer Berlin [22] G.P., Mack M. and Contiero J. (2009) ‎Biotechnol Adv, 27(1), [23] P., Sharma R., Ray S., Mehariya S., Patel S.K., Lee J.K. and Kalia V.C. (2015) Bioresour Technol, 182, [24] T., Rao Z., Zhang X., Xu M., Xu Z. and Yang S.T. (2015) Microb Cell Fact, 14(1), 122-133. [25] Y., Murarka A. and Gonzalez R. (2006) ‎Biotechnol Bioeng, 94(5), [26] S. and Devaraj A. (2013) IJSRP, 3(8), [27] R.K., Anand P., Saran S. and Isar J. (2009) Biotechnol Adv, 27(6), [28] D. (2015) Electronic Electron J Biotechnol, 18(2), [29] T., Nakashimada Y., Senba K., Matsui T. and Nishio N. (2005) J BioscI Bioeng, 100(3), [30] B., Bahl H. and Gottschalk G. (1992) Appl Environ Microbiol, 58(4), [31] III J.J. (1999) In: Murray P.R., Baron E.J., Pfaller M.A., Tenover F.C., Yolken R.H. (ed) Manual of clinical microbiology, 7 edn. Washington D.C., ASM [32] G. A. (2017). Engineering of Microorganisms for the Production of Chemicals and Biofuels from Renewable Resources. Springer International [33] L.F., Arcaro J.R.P. and Nader Filho A. (2009) Ci Anim Bras, 10 (4), [34] J.R. (2007) Dissertation, Federal University of Santa Catarina [35] K. and Petrova P. (2010) Appl Microbiol Biotechnol, 87(3), [36] G., Yang G., Wang X., Guo Q., Li Y. and Li J. (2012) Appl Biochem Biotechnol, 168(1), [37] H.W., Li F.T. and Chang J.S. (2014) Bioresour Technol, 153, [38] S., Prasertsan P. and Methacanon P. (2011) Process Biochem, 46(2), 608-614.