ASSESSMENT OF THE BACTERIOLOGICAL QUALITY OF THE RAW WATER AND THE ANTIMICROBIAL SUSCEPTIBILITY PROFILE OF BACTERIA ISOLATED IN WATER SURFACE OF A RIVER

Raylane Pereira Gomes¹*, Ariana Alves Rodrigues², Marcia Regina Pincerati³, Monica Santiago Barbosa⁴, Carla Afonso da Silva Bitencourt Braga⁵, Jose Daniel Goncalves Vieira⁶, Lilian Carla Carneiro^7
1IPTSP Department, Federal University of Goias, Goiania, Brazil
²IPTSP Department, Federal University of Goias, Goiania, Brazil
³University Positive, Curitiba, Parana, Brazil
⁴IPTSP Department, Federal University of Goias, Goiania, Brazil
⁵IPTSP Department, Federal University of Goias, Goiania, Brazil
⁶IPTSP Department, Federal University of Goias, Goiania, Brazil
⁷University Positive, Curitiba, Parana, Brazil
* Corresponding Author : carlacarneirolilian@gmail.com

Received : 18-08-2017     Accepted : 07-09-2017     Published : 28-09-2017
Volume : 9     Issue : 9       Pages : 949 - 953
Int J Microbiol Res 9.9 (2017):949-953

Keywords : Resistance, Antibiotics, Contaminants, Aquatic environment, Bacteria
Academic Editor : K.S.V.P. Chandrika, Dr P. Katyal, Marcos Antônio Pesquero, Ashish Kumar, D. G. Panpatte, R. Rani, Leishangthem Chanu Langlentombi, Erika Izumi, Dr V Kasthuri Thilagam
Conflict of Interest : None declared
Acknowledgements/Funding : CAPES (Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior) by scholarship to student
Author Contribution : All author equally contributed

Antibiotic resistance in the aquatic environment can originate from contaminants of human and animal origin such as waste, sewage, effluents, chemical and pharmaceutical products. These contaminants may select resistant organisms and/or introduce resistant bacteria directly in this environment. Thus, the aquatic environment acts as a reservoir for the spread of multidrug-resistant bacteria. The aim of this study was to evaluate the microbiological contamination and the level of antibiotic resistance in isolates from samples of raw surface water of the Meia Ponte River, in Goiás State, Brazil. It was performed the multiple-tube fermentation technique to quantify bacterial contamination of the water samples from the river, biochemical tests for the identification of bacterial isolates, and disk diffusion susceptibility test to check sensitivity to antimicrobial agents. It was found high counts of bacteria in the coliform group, mainly during rainy periods. Of the 103 isolated bacteria, the majority was capable of transmitting diseases, mostly gastroenteritis. In addition, it was observed a worrying rate of resistant bacteria present in the environment. Further researches are necessary to understand the demand of antibiotic resistance in the aquatic environment, and to verify its consequences. Some measures should be created to prevent the spread of resistant microorganisms in the environment.

1. Jordaan K. and Bezuidenhout C.C. (2016) Environmental Science and Pollution Research, 23(6), 5868-5880.
2. Korajkic A., Parfrey L.W., McMinn B.R., Baeza Y.V., VanTeuren W., Knight R. and Shanks O.C. (2015) Water Research, 69, 30-39.
3. Pandey P.K., Kass P.H., Soupir M.L., Biswas S. and Singh V.P. (2014) Amb Express, 4(1), 51.
4. Musyoki A.M., Mbaruk A.S., Mbithi J.N. and Maingi J.M. (2013) International Journal of Life Science and Pharma Research, 3(1), 04-10.
5. Schreiber C., Rechenburg A., Rind E. and Kistemann T. (2015) International Journal of Hygiene and Environmental Health, 218(2), 181-187.
6. Vaz-Moreira I., Nunes O.C. and Manaia C.M. (2014) FEMS Microbiology Reviews,38(4), 761-778.
7. Koczura R., Krysiak N., Taraszewska A. and Mokracka J. (2015) Journal of Applied Microbiology, 119(2), 594-603.
8. Narciso-da-Rocha C. and Manaia C.M. (2016) Science of the Total Environment, 563: 1-9.
9. Zhao Z., Wang J., Han Y., Chen J., Liu G., Lu H., Yan B. and Chen S. (2017) Environmental Pollution, 220, 909-918.
10. Manaia C.M., Macedo G., Fatta-Kassinos D. and Nunes O.C. (2016) Applied Microbiology Biotechnology, 100(4), 1543-1557.
11. Yidong G.U.A.N., Bo W.A.N.G., Yongxia G.A.O., Wen L.I.U., Xiaoli Z.H.A.O., Huang X. and Jianghua Y.U. (2017). Pedosphere, 27(1), 42-51.
12. Marti E., Variatza E. and Balcazar J.L. (2014) Current Trends in Microbiology, 22(1), 36-41.
13. Sood N.K., Patel P.C., Patel S.M. and Mandalia A.H. (2015) International Journal of Medical Science and Public Health, 4(12), 1739-1744.
14. Al-Bahry S.N., Paulson J.R. and Al-Musharaf S.K. (2014) The International Arabic Journal of Antimicrobial Agents, 4(4), 1-11.
15. Vaz-Moreira I., Varela A.R., Pereira T.V., Fochat R.C. and Manaia C.M. (2015) Microbial Drug Resistance, 00, 01-09.
16. Brandão C.J., Botelho M.J.C., Sato M.I.Z. and Lamparelli M.C. (2011) http://www.cqa.com.br/arquivos/Guia_Nacional_de_Coleta-CETESB-ANA-2012.pdf of subordinate document. Accessed 28 Jan 2017.
17. American Public Health Association, et al. Standard methods for the examination of water and wastewater. 22 ed. American Public Health Association; 2012.
18. National Sanitary Surveillance Agency, Brazil. (2013) http://www20.anvisa.gov.br/segurancadopaciente/index.php/publicacoes/item/deteccao-e-identificacao-de-bacterias-de-importancia-medica of subordinate document. Accessed 28 July 2017.
19. Hudzicki J. (2009) http://www.asmscience.org/docserver/fulltext/education/protocol/protocol.3189.pdf?expires=1501266906&id=id&accname=guest&checksum=AA2369E4E9A05B8DD71696B85F47F8D8 of subordinate document. Accessed 20 Jan 2015.
20. Clinical & Laboratory Standards Institute. (2016) 26th Performance Standards for Antimicrobial Susceptibility Testing ed. CLSI supplement M100S. Wayne, PA: Clinical and Laboratory Standards Institute, 1-256.
21. StatSoft, I. (2012). STATISTICA (data analysis software system), version 7. 2004. Tulsa, USA, 150.
22. Fonseca R.C., Souza N.A.D., Correa T.C.L, Garcia LF., Reis L.G.V.D. and Rodriguez A.G. (2013) Food Science and Technology (Campinas), 33(2), 251-256.
23. Sivaraja R. and Nagarajan K. (2014) International Journal of PharmTech Research, 6(2), 455-461.
24. Laurent S.J. and Mazumder A. (2014) Water Research, 48, 170-178.
25. Abia A.L.K., Ubomba-Jaswa E., Genthe B. and Momba M.N.B. (2016) Science of the Total Environment, 566, 1143-1151.
26. Holvoet K., Sampers I., Seynnaeve M. and Uyttendaele M. (2014) International Journal of Food Microbiology, 171, 21-31.
27. Bouki C., Venieri D. and Diamadopoulos E. (2013) Ecotoxicology and Environmental Safety, 91, 1-9.
28. Finley R.L., Collignon P., Larsson D.J., McEwen S.A., Li X.Z., Gaze W.H., Reid-Smith R., Timinouni M., Graham D.W. and Topp E. (2013) Clinical Infectious Diseases, 6, 01-07.
29. Varela A.R. and Manaia C.M. (2013) Environmental Science and Pollution Research, 20(6), 3550-3569.
30. Wellington E.M., Boxall A.B.A., Cross P., Feil E.J., Gaze W.H., Hawkey P.M., Johnson-Rollings A.S., Jones D.L., Lee N.M., Otten W., Thomas C.M. and PrysorWilliams A. (2013) The Lancet Infectious Diseases, 13(2), 155-165.
31. Islam M.S., Ahmed M.K., Raknuzzaman M., Habibullah-Al-Mamun M. and Islam M.K. (2015) Ecological Indicator, 48, 282-291.
32. Khan S., Shahnaz M., Jehan N., Rehman S., Shah M.T. and Din I. (2013) Journal of Clean Production, 60, 93-101.
33. Lin M., Liang J., Zhang X., Wu X., Yan Q. and Luo Z. (2015) Environmental Science and Pollution Research, 22(15), 11930-11939.
34. Yao L., Wang Y., Tong L., Deng Y., Li Y., Gan Y., Guo W., Dong C., Duan Y. and Zhao K. (2017) Ecotoxicology and Environmental Safety, 135, 236-242.

35. Alipour M., Hajiesmaili R., Talebjannat M. and Yahyapour Y. (2014) The Scientific World Journal, 1-5.
36. Lobova T.I., Yemelyanova E., Andreeva I.S., Puchkova L.I. and Repin V.Y. (2015) Microbial Drug Resistance, 21(4), 477-490.
37. Kumar A., Verma A.K., Gupta M.K. and Rahal A. (2014) Pak Journal of Biological Scice, 17(4), 540-544.
38. Skariyachan S., Mahajanakatti A.B., Grandhi N.J., Prasanna A., Sen B., Sharma N., Vasist K.S. and Narayanappa R. (2015) Environmental Monitoring and Assessment, 187(5), 279.
39. Titilawo Y., Sibanda T., Obi L. and Okoh A. (2015) Environmental Science and Pollution Research, 22(14), 10969-10980.
40. Narciso-da-Rocha C. and Manaia C.M. (2017) Science of the Total Environment, 575, 932-940.
41. Walia S., Murleedharn C., Band J., Kanwar M. and Kumar A. (2016) Current Medicine Research and Practice, 6(4), 149-151.
42. Erhan H.E., Gill K., Swann S. and Lam K. (2016) Simon Fraser university Science Undergraduate Research Journal, 1, 01-11.
43. Gómez P., Casado C., Sáenz Y., Ruiz-Ripa L., Estepa V., Zarazaga M. and Torres C. (2017) FEMS Microbiology Ecology, 93(1), fiw208.
44. Nakayama T., Hoa T.T.T., Harada K., Warisaya M., Asayama M., Hinenoya A., Lee J.W., Phu T.M., Euda S., Sumimura Y., Hirata K., Phuong N.T. and Yamamoto Y. (2017) Environmental Pollution, 222, 294-306.
45. Rocha M.P., Dourado P.L.R., Rodrigues M.S., Raposo J.L., Grisolia A.B. and Oliveira K.M.P. (2015) Environmental Monitoring and Assessment, 7(187), 1-12.
46. Conte D., Palmeiro J.K., da Silva Nogueira K., de Lima T.M.R., Cardoso M.A., Pontarolo R., Pontes F.L.D. and Dalla-Costa L.M. (2017) Ecotoxicology and Environmental Safety, 136, 62-69.
47. Coutinho F.H., Silveira C.B., Pinto L.H., Salloto G.R., Cardoso A.M., Martins O.B., Vieira R.P. and Clementino M.M. (2014) Microbial Ecology, 68(3), 441-452.
48. Su H.C., Ying G.G., Tao R., Zhang R.Q., Zhao J.L. and Liu Y.S. (2012) Environmental Pollution, 169, 42-49.
49. Berendonk T.U., Manaia C.M., Merlin C., Fatta-Kassinos D., Cytryn E., Walsh F., Burgmann H., Sorum H., Norstrom M., Pons M., Kreuzinger N., Huovinen P., Stefani S., Schwartz T., Kisand V., Baquero F. and Martinez J.L. (2015) Nature Reviews Microbiology,13(5), 310-317.