ISSN : 0975-5276
EISSN : 0975-9174
NISH KUMAR SHARMA1*, JYOTSANA PANDIT2, POONAM SHIRKOT3
1School of Biotechnology, P P Savani University, Kosamba, Surat, 394125, India
2Department of Environmental Science, Dr Y S Parmar University of Horticulture and Forestry, Nauni, Solan, 173 230, India
3Department of Biotechnology, Dr Y S Parmar University of Horticulture and Forestry, Nauni, Solan, 173 230, India
* Corresponding Author : janish.sharma28@gmail.com
Received : 17-08-2017 Accepted : 07-09-2017 Published : 28-09-2017
Volume : 9 Issue : 9 Pages : 941 - 945
Int J Microbiol Res 9.9 (2017):941-945
Keywords : Chlorpyrifos, Gas Chromatography (GC), Flame Ionization Detector (FID), Physico-chemical Properties
Academic Editor : Azza Mohamed Said Zakaria, Shanthi N, Dr Surabhi Yadav
Conflict of Interest : None declared
Acknowledgements/Funding : The author acknowledges the financial support from the Dean, College of Horticulture, Dr Y.S. Parmar University of Horticulture and Forestry, Solan (H.P.). Author thanks Jyotsana Pandit, Research Scholar for assistance with soil physico-chemical properties studies and Dr Poonam Shirkot, Professor Biotechnology, Dr Y.S. Parmar University of Horticulture and Forestry, Solan for valuable comments and guidance
Author Contribution : All authors had equally contributed
Samples of soil from apple orchards with previous history of continuous and extensive use of chlorpyrifos were collected. Chlorpyrifos residues were determined in soil samples collected from Shimla and Kullu districts of Himachal Pradesh. Residues of Chlorpyrifos were estimated by GC equipped with FID system. A higher percentage recovery of chlorpyrifos was observed (90.80-93.60%) in Kullu district soil samples than Shimla district samples. The pH and electrical conductivity (EC) of the soil samples from apple orchards were recorded and ranged from 6.66-7.23 and 0.27-0.37 dSm-1 respectively. The percent organic carbon, nitrogen, phosphorus and potassium content of soil samples ranged from 0.69-1.12%, 312.67-479.67 Kg/ha, 13.37-23.20 Kg/ha and 261.67-365.67 Kg/ha respectively. Mesophilic bacterial population colonizing various apple orchard soil samples were enumerated after 48 hrs of incubation on nutrient agar at 37°C using serial dilution technique and it was observed that bacterial population in the samples ranged between 0.78 x 105 to 2.41 x 105 cfu/g. A total of seventy two different bacterial strains were further isolated and out of them only fifteen bacterial stains exhibited chlorpyrifos degrading potential. In our study we monitored chlorpyrifos residues along with the physico-chemical analysis of soil samples and also investigated indigenous bacteria inhabiting the apple orchard soils with continuous exposure to chlorpyrifos.
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