INDUCED GENETIC VARIABILITY AND DIVERGENCE THROUGH PHYSICAL AND CHEMICAL MUTAGENS IN M3 GENERATION OF GREENGRAM (Vigna radiata L. WILCZEK)

PRONOB J. PAUL¹, G. ROOPA LAVANYA²*, G. SURESH BABU³, YUVRAJ YADAV⁴, UMAKANT VERMA^5
1Department of Genetics and Plant Breeding, Sam Higginbottom University of Agriculture, Technology and Sciences, Allahabad, 211007, Uttar Pradesh, India
²Department of Genetics and Plant Breeding, Sam Higginbottom University of Agriculture, Technology and Sciences, Allahabad, 211007, Uttar Pradesh, India
³Department of Genetics and Plant Breeding, Sam Higginbottom University of Agriculture, Technology and Sciences, Allahabad, 211007, Uttar Pradesh, India
⁴Department of Genetics and Plant Breeding, Sam Higginbottom University of Agriculture, Technology and Sciences, Allahabad, 211007, Uttar Pradesh, India
⁵Department of Genetics and Plant Breeding, Sam Higginbottom University of Agriculture, Technology and Sciences, Allahabad, 211007, Uttar Pradesh, India
* Corresponding Author : lavanya.roopa@gmail.com

Received : 16-06-2017     Accepted : 25-06-2017     Published : 28-06-2017
Volume : 9     Issue : 6       Pages : 284 - 286
Genetics 9.6 (2017):284-286

Keywords : Greengram, Variability, Genetic divergence, Inter-cluster distance
Academic Editor : Ketan Govindbhai Kanjariya, Shilpa Gupta
Conflict of Interest : None declared
Acknowledgements/Funding : The authors duly acknowledge the Gamma irradiation facility provided for the experimental materials by the CSIR- National Botanical Research Institute (NBRI), Lucknow, Uttar Pradesh 226001, India
Author Contribution : GRL and PJP conceived this study. UV, YY, GRL helped PJP in field experiments and phenotyping. PJP, GRL and GSB interpreted the data. PJP and GRL wrote the manuscript and GSB, UV and YY contributed to it. All the authors reviewed and approved the final ma

An experiment was carried out to evaluate mutant population for variability and divergence in M3 generation during kharif 2009. High magnitude of phenotypic coefficient of variation (PCV), as well as genotypic coefficient of variation (GCV), high heritability coupled with high genetic advance as percent of mean, were recorded for number of clusters per plant, number of primary branches, seed yield per plant and number of pods per plants, suggesting additive gene effects and selection may be effective for these characters for yield improvement. Fifty mutant lines along with a parent, formed eight clusters where cluster VII emerged as the largest one comprising 10 mutant lines and the cluster VIII as the smallest one containing three mutant lines. The maximum inter-cluster distance was observed between clusters I and VIII, suggesting that the selection of parents for hybridization from the diverse clusters to get a broad spectrum of variability. Estimation of genetic divergence among mutant lines in M3 generation has immense bearing on identifying potential mutant lines, which may produce a broad spectrum of variability with transgressive segregants following hybridization.

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