ISSN : 0975-5276
EISSN : 0975-9174
NEHA MISHRA1*, DILEEP K. SINGH2
1Department of Zoology, University of Delhi, New Delhi, Delhi 110021
2Department of Zoology, University of Delhi, New Delhi, Delhi 110021
* Corresponding Author : nehamishra2706@gmail.com
Received : 06-06-2017 Accepted : 23-06-2017 Published : 28-07-2017
Volume : 9 Issue : 7 Pages : 909 - 912
Int J Microbiol Res 9.7 (2017):909-912
Keywords : Achromobacter, biodegradation, bioremediation, DDT, Yamuna
Academic Editor : Dr Sumit Pal, Dr Dhruba Jyoti Sarkar
Conflict of Interest : None declared
Acknowledgements/Funding : Financial assistance provided by NASF, Indian Council of Agricultural Research, New Delhi is greatly acknowledged
Author Contribution : All author equally contributed
Ubiquitous presence of DDT owing to its excessive use and recalcitrance has been a matter of concern worldwide. The current study is an attempt to address this problem by isolating a bacterium possessing the capability of utilizing DDT as a sole Carbon source by selective enrichment technique. The isolated strain was identified to belong to bacteria Achromobacter on the basis of 16S rRNA gene similarity. The study revealed that strain Y12A was the most efficient degrader, degrading 81.25% DDT within 10 days of incubation. The degrading ability of the strain escalated at optimum conditions of 30ºC temperature, pH 7 and 50 mgL-1 of DDT concentration. Metabolites obtained during DDT degradation were DDE, DDD and DDMU. Hence, DDT degrading efficiency of the isolated strain Y12A could be utilized in integration with DDMU utilizing strains in consortia to achieve mineralization of DDT. To the best of our knowledge, this is the first instance when member of Achromobacter genus has been reported to possess DDT degrading ability. The study reveals the degradation potential of Achromobacter sp. strain Y12A for the bioremediation of DDT from polluted sites.
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